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. 2012 Nov 14;7(1):32.
doi: 10.1186/1750-9378-7-32.

The SNP at -592 of human IL-10 gene is associated with serum IL-10 levels and increased risk for human papillomavirus cervical lesion development (VSports注册入口)

Kirvis Torres-Poveda  1 , Ana I Burguete-García, Miguel Cruz, Gabriela A Martínez-Nava, Margarita Bahena-Román, Esmeralda Ortíz-Flores, Abrahan Ramírez-González, Guillermina López-Estrada, Karina Delgado-Romero, Vicente Madrid-Marina
Affiliations

The SNP at -592 of human IL-10 gene is associated with serum IL-10 levels and increased risk for human papillomavirus cervical lesion development

Kirvis Torres-Poveda et al. Infect Agent Cancer. .

Abstract

Background: Women with Human Papilloma Virus (HPV) persistence are characterized by high levels of IL-10 at cervix. We have determined whether polymorphisms of IL-10 gene promoter might be associated with increased risk of squamous intraepithelial cervical lesions (SICL) and whether exist significative differences of IL-10 mRNA expression at cervix and systemic and serum IL-10 protein between SICL cases and non-Cervical Lesions (NCL). VSports手机版.

Methods: Peripheral blood samples from SICL (n = 204) and NCL (n = 166) were used to detect IL-10 promoter polymorphisms at loci -592A/C (rs1800872), -819C/T (rs1800871), -1082A/G (rs1800896), -1352A/G (rs1800893), by allelic discrimination and to evaluate serum IL-10 protein. Cervical epithelial scrapings from NCL and biopsies from SICLs were used for HPV-typing and to evaluate IL-10 mRNA expression level. The systemic and local IL-10 mRNA expression levels were measured by real time-PCR V体育安卓版. Genotypic and allelic frequencies of the selected polymorphisms were analyzed by logistic regression, adjusting by age and HPV-genotype, to determine the association with SICL. .

Results: No significant differences were found between genotype frequencies at loci -819, -1082, and -1352. Individuals carrying at least one copy of risk allele A of polymorphism -592 had a two-fold increased risk of developing SICL [adjusted odds ratio (OR), 2. 02 (95% CI, 1. 26-3. 25), p = 0. 003], compared to NCL V体育ios版. The IL-10 mRNA expression and serum IL-10 protein, were significantly higher in SICL cases (p < 0. 01), being higher in patients carrying the risk allele A. .

Conclusions: The -592 polymorphism is associated with increased risk of SICL and can serve as a marker of genetic susceptibility to SICL among Mexican women. According to IL-10 levels found in SICL, IL-10 can be relevant factor for viral persistence and progression disease VSports最新版本. .

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Figures

Figure 1
Figure 1
IL-10 mRNA levels normalized to GAPDH and HPRT1 at the systemic level and in cervix, in NCL and SICL. The levels were higher in SICL compared to NCL, at the systemic level and in cervix, p < 0.0001.
Figure 2
Figure 2
IL-10 protein serum levels in women with SICL versus NCL. The levels were higher in SICL compared to NCL, p < 0.0001.
See this image and copyright information in PMC

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