Objective: Using highly sensitive polymerase chain reaction (PCR) techniques, we reanalysed all cervical carcinomas (CCs) found to be human papillomavirus (HPV)-negative by Hybrid Capture 2 (HC2) to determine the prevalence of true HPV-negativity. We also evaluated the characteristics of the patients with tumours with confirmed HPV-negativity. VSports手机版.

Design: Observational study V体育安卓版. .

Setting: Barcelona, Spain. V体育ios版.

Population: A cohort of 136 women with CC (32 adenocarcinomas, 104 squamous cell carcinomas) who had pre-treatment HC2 testing VSports最新版本. .

Methods: All negative cases were reanalysed and genotyped for HPV using three PCR assays (SPF10, GP5+/6+ and E7-specific assay). V体育平台登录.

Main outcome measures: Percentage of confirmed HPV-negative and HPV-positive tumours VSports注册入口. Clinicopathological features and disease-free survival (DFS) and overall survival (OS) of both groups. .

Results: Fourteen of 136 women (10. 2%) were negative for HPV by HC2. After reanalysis by PCR-based techniques only 8/136 (5. 8%) tumours were confirmed as HPV-negative, whereas in six cases different HPVs were identified [HPV-11, -16 (two tumours), -18, -45, and -68]. Confirmed HPV-negativity was more frequent in adenocarcinomas than in squamous cell carcinomas (5/32, 15. 6% versus 3/104, 2. 9%, respectively; P = 0. 017). Patients with CCs with confirmed HPV-negativity had significantly worse DFS than women with HPV-positive tumours [51. 9 months (95% CI 12. 2-91. 7 months) versus 109. 9 months (95% CI 98. 2-121. 5 months); P = 0. 010]. In the multivariate analysis HPV-negativity and International Federation of Gynecology and Obstetrics (FIGO) staging were associated with increased risk of progression and mortality V体育官网入口. .

Conclusions: An HC2-negative result is an uncommon finding in women with CC, but in almost half of these cases HPVs are identified by more sensitive techniques VSports在线直播. CCs with confirmed HPV-negativity are more frequently adenocarcinomas, and seem to be associated with worse DFS. .