Reference: Johnson RE, et al. (1999) Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Poleta. Science 283(5404):1001-4

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Abstract


The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation VSports app下载. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase eta.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Johnson RE, Prakash S, Prakash L
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