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. 2021 Feb;38(2):301-317.
doi: 10.1007/s11095-021-02989-y. Epub 2021 Feb 19.

Folic Acid-Doxorubicin-Double-Functionalized-Lipid-Core Nanocapsules: Synthesis, Chemical Structure Elucidation, and Cytotoxicity Evaluation on Ovarian (OVCAR-3) and Bladder (T24) Cancer Cell Lines

Rodrigo Cé  1 , Vladimir Lavayen  2 , Gabriela Klein Couto  3 , João Guilherme Barreto De Marchi  4 , Barbara Zoche Pacheco  5 , Letícia Antunes Natividade  5 , Tiago Ost Fracari  2 , Taiane Medeiro Ciocheta  4 , Aline de Cristo Soares Alves  4 , Denise Soledade Jornada  4 , Silvia Stanisçuaski Guterres  4 , Fabiana Seixas  3 , Tiago Collares  3 , Adriana Raffin Pohlmann  6   7
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"V体育2025版" Folic Acid-Doxorubicin-Double-Functionalized-Lipid-Core Nanocapsules: Synthesis, Chemical Structure Elucidation, and Cytotoxicity Evaluation on Ovarian (OVCAR-3) and Bladder (T24) Cancer Cell Lines

Rodrigo Cé et al. Pharm Res. 2021 Feb.

Abstract

Purpose: Folic acid-doxorubicin-double-functionalized-lipid-core nanocapsules (LNC-CS-L-Zn+2-DOX-FA) were prepared, characterized, and evaluated in vitro against ovarian and bladder cancer cell lines (OVCAR-3 and T24). VSports手机版.

Methods: LNC-CS-L-Zn+2-DOX-FA was prepared by self-assembly and interfacial reactions, and characterized using liquid chromatography, particle sizing, transmission electron microscopy, and infrared spectroscopy. Cell viability and cellular uptake were studied using MTT assay and confocal microscopy V体育安卓版. .

Results: The presence of lecithin allows the formation of nanocapsules with a lower tendency of agglomeration, narrower size distributions, and smaller diameters due to an increase in hydrogen bonds at the surface. LNC-L-CS-Zn+2-DOX-FA, containing 98. 00 ± 2. 34 μg mL-1 of DOX and 105. 00 ± 2. 05 μg mL-1 of FA, had a mean diameter of 123 ± 4 nm and zeta potential of +12. 0 ± 1. 3 mV. After treatment with LNC-L-CS-Zn+2-DOX-FA (15 μmol L-1 of DOX), T24 cells had inhibition rates above 80% (24 h) and 90% (48 h), whereas OVCAR-3 cells showed inhibition rates of 68% (24 h) and 93% (48 h), showing higher cytotoxicity than DOX. HCl V体育ios版. The fluorescent-labeled formulation showed a higher capacity of internalization in OVCAR-3 compared to T24 cancer cells. .

Conclusion: Lecithin favored the increase of hydrogen bonds at the surface, leading to a lower tendency of agglomeration for nanocapsules VSports最新版本. LNC-CS-L-Zn+2-DOX-FA is a promising therapeutic agent against tumor-overexpressing folate receptors. .

Keywords: OVCAR-3: ovarian cancer line; T24: bladder cancer line; doxorubicin; folic acid; lipid-core nanocapsules. V体育平台登录.

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