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. 2019 Oct 8;3(19):2866-2869.
doi: 10.1182/bloodadvances.2019000362.

Butyrogenic bacteria after acute graft-versus-host disease (GVHD) are associated with the development of steroid-refractory GVHD

Jonathan L Golob  1 , Martha M DeMeules  2 , Tillie Loeffelholz  2 , Z Z Quinn  2 , Michael K Dame  3 , Sabrina S Silvestri  3 , Michael C Wu  4 , Thomas M Schmidt  1 , Tina L Fiedler  2 , Matthew J Hoostal  1 , Marco Mielcarek  5   6 , Jason Spence  3   7   8 , Steven A Pergam  2   5   9 , David N Fredricks  2   5   9
Affiliations

Butyrogenic bacteria after acute graft-versus-host disease (GVHD) are associated with the development of steroid-refractory GVHD

Jonathan L Golob (V体育官网) et al. Blood Adv. .

Abstract (VSports手机版)

  1. The presence of butyrogenic bacteria after the onset of acute GVHD associates with subsequent steroid-refractory GVHD or chronic GVHD.

  2. Butyrate inhibits human colonic stem cells from forming an intact epithelial monolayer.

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Conflict of interest statement

Conflict-of-interest disclosure: S. A. P. has received research support from Global Health Technologies, Inc. and participates in clinical trials with Chimerix. The remaining authors declare no competing financial interests V体育ios版.

Figures

Figure 1.
Figure 1.
The relative abundance and prevalence (fraction of HCT recipients with at least 1 butyrogen present) of butyrogens after the onset of acute GVHD of the gut stratified by steroid responsive or refractory GVHD. All plots are rolling averages. P values from mixed-effects modeling from day 0 to +21 (with gray background) relative to the peak of acute GVHD symptoms. Black dashed line is the median value for healthy donors.
Figure 2.
Figure 2.
Transepithelial electrical resistance (TEER, in ohms * cm2) in differentiating colonic epithelium derived from primary human colonoids with varying timing of butyrate in the luminal compartment. With Hanks Buffered Salt Solution in the luminal compartment (No SCFA), a high level of TEER is achieved by differentiation day 4. With HBSS with 10 mM butyrate (Butyrate-Early) in the luminal compartment from day 1 onward, TEER does not develop. Butyrate (10 mM) from day 3 onward (Butyrate-Late) does not impair the development of TEER. Points are offset on the x-axis from the ordinal days for visual clarity.
See this image and copyright information in PMC

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