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. 2018 Sep 18;28(2):547-553.
doi: 10.1007/s10068-018-0470-6. eCollection 2019 Apr.

Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1β via an anti-inflammatory mechanism

Leeseon Kim  1 , Ji Yeon Kim  1
Affiliations

Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1β via an anti-inflammatory mechanism

"V体育安卓版" Leeseon Kim et al. Food Sci Biotechnol. .

Abstract

A complex of curcumin and lecithin developed to improve the solubility of curcumin, enhanced its chondroprotective effect via an anti-inflammatory mechanism. In macrophage, proinflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, prostaglandin E2 (PGE2), and nitric oxide (NO) were quantified. In addition, the activity of nuclear factor (NF)-κB was examined. With chondrocytes, inflammatory mediators were assessed by measuring the secretion levels of IL-6, IL-8, and PGE2, also the mRNA expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Metalloproteinases (MMPs), tissue inhibitor of metalloprotease (TIMP)-1, type II collagen (COL2), proteoglycan (PG), and hyaluronic acid (HA) were measured with respect to the articulation surface. The complex promoted the anti-inflammatory effect by the inhibition of inflammatory mediators. In addition, mRNA expression levels ameliorated. Furthermore, it was effective in decreasing extracellular secretion of polypeptides, also corresponding intracellular MMPs and TIMP-1 VSports手机版. In conclusion, the complex may be developed as a functional supplement to maintain articulation health. .

Keywords: Ant-inflammatory effect; Chondroprotective effect; Curcumin; Lecithin complex; Osteoarthritis. V体育安卓版.

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Figures

Fig. 1
Fig. 1
NF-κB activity (A); comparison of TNF-α, IL-1β, IL-6, and PGE2 (B); concentration profile of NO (C) production; relative mRNA expression levels of the genes COX-2 and iNOS (D) in Raw 264.7 cells stimulated with LPS. Cells were seeded at 1.0 × 105 cells/mL, then stimulated with LPS and sample for 48 h. To calculate the relative mRNA expression levels, the ΔΔCT method was used. The mRNA levels were normalized by β-actin. *Significant different from the LPS values by ANOVA followed by Dunnett’s test (p < 0.05)
Fig. 2
Fig. 2
Comparison of COL2 (A), PG (B), HA (C), and TIMP-1 (D) in HCH-c cells stimulated with IL-1β. Cells were seeded at 1.0 × 105 cells/mL, then stimulated with IL-1β and sample for 48 h. * Significant different from the IL-1β values by ANOVA followed by Dunnett’s test (p < 0.05)
Fig. 3
Fig. 3
Comparison (A) and relative hepatic mRNA expression levels (B) of MMP-1, 2, 3, 9, and 13 in HCH-c cells stimulated with IL-1β. Cells were seeded at 1.0 × 105 cells/mL, then stimulated with IL-1β and sample for 48 h. To calculate the relative mRNA expression levels, the ΔΔCT method was used. The mRNA levels were normalized by β-actin. *Significant different from the IL-1β values by ANOVA followed by Dunnett’s test (p < 0.05)
Fig. 4
Fig. 4
Relative hepatic mRNA expression levels (A) of the genes COX-2, iNOS and comparison (B) of IL-6, IL-8, PGE2 in HCH-c cells stimulated with IL-1β. Cells were seeded at 1.0 × 105 cells/mL, then stimulated with IL-1β and sample for 48 h. To calculate the relative mRNA expression levels, the ΔΔCT method was used. The mRNA levels were normalized by β-actin. *Significant different from the IL-1β values by ANOVA followed by Dunnett’s test (p < 0.05)
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