日
 Skip to main page content (VSports手机版)
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

Review
. 2016 Nov;24(11):887-901.
doi: 10.1016/j.tim.2016.06.008. Epub 2016 Jul 15.

Emerging Technologies for Gut Microbiome Research

Jason W Arnold  1 , Jeffrey Roach  2 , M Andrea Azcarate-Peril  3
Affiliations
Review

Emerging Technologies for Gut Microbiome Research

Jason W Arnold et al. Trends Microbiol. 2016 Nov.

Abstract

Understanding the importance of the gut microbiome on modulation of host health has become a subject of great interest for researchers across disciplines VSports手机版. As an intrinsically multidisciplinary field, microbiome research has been able to reap the benefits of technological advancements in systems and synthetic biology, biomaterials engineering, and traditional microbiology. Gut microbiome research has been revolutionized by high-throughput sequencing technology, permitting compositional and functional analyses that were previously an unrealistic undertaking. Emerging technologies, including engineered organoids derived from human stem cells, high-throughput culturing, and microfluidics assays allowing for the introduction of novel approaches, will improve the efficiency and quality of microbiome research. Here, we discuss emerging technologies and their potential impact on gut microbiome studies. .

Keywords: automated culturing; gut microbiome; high-throughput sequencing; microbial cultivation; personalized medicine. V体育安卓版.

PubMed Disclaimer

Figures

Figure I
Figure I. A Microbiome-Organoid Array to Study the Impact of Gut Microbiome Modulators on Individual Hosts
A) Host-derived organoid arrays could derive personalized treatments and diagnostics based on genetic background (GB) and gut microbiome composition. Individual organoids can subsequently be harvested and screened for microbial composition, microbial transcriptome, and host gene expression with the aim of identifying an effective treatment for the individual. B) Mouse small intestine stem cell-derived enteroid from days 4 to 7. Photographs show progression of the organoid from a sphere to mature colonoids presenting crypt-like structures.
Figure 1
Figure 1
Timeline of major advancements on gut microbiota studies. Research centered around low-cost high-throughput next generation sequencing began to emerge in the past 10 years. These advancements have provided the opportunity to study complex communities and their impact on host health, and culmination of these technologies is driving the field toward a future of personalized medicine. Taxonomic determination of microbes dwelling in the gut by 16S rRNA sequencing was first performed in the late 1990s. Dysbiosis and its impact on human disease was first discussed in early 2000s, and identified in colorectal cancer [133] among other diseases including Crohn's disease. Since 2010, rapid advances in bioinformatics including Quantitave Insights into Microbial Ecology (QIIME) for analysis of metagenome have been used to identify core members of the gut microbiota of healthy humans [134] as well as the impact of perturbations on gut microbial communities [135]. Finally, biology and engineering sciences converged in the design of ‘Gut on a Chip’ devices to simulate gastrointestinal environments [70].
See this image and copyright information in PMC

V体育官网入口 - References

    1. Savage DC. Microbial ecology of the gastrointestinal tract. Annu Rev Microbiol. 1977;31:107–33. - PubMed
    1. de Vos W, de Vos E. Role of the intestinal microbiome in health and disease: from correlation to causation. Nutr Rev. 2012;70:45–56. - PubMed
    1. Kau AL, et al. Human nutrition, the gut microbiome and the immune system. Nature. 2011;474(7351):327–36. - PMC - PubMed
    1. Sobhani I, et al. Microbial dysbiosis in colorectal cancer (CRC) patients. PLoS One. 2011;6(1):e16393. - PMC - PubMed
    1. Carding S, et al. Dysbiosis of the gut microbiota in disease. Microb Ecol Health Dis. 2015;26:26191. - "V体育官网" PMC - PubMed

Publication types

Substances