VSports - Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2015 Apr;27(2):157-62.
doi: 10.5021/ad.2015.27.2.157. Epub 2015 Mar 24.

Identification of leukocyte-specific protein 1-positive cells: a clue to the cell of origin and a marker for the diagnosis of dermatofibroma

Sang Yun Jin  1 , Jong Sun Choi  2 , Yoon La Choi  3 , Yoon La Choi  3 , Do Hun Kim  1 , Seung Ho Lee  1
Affiliations

Identification of leukocyte-specific protein 1-positive cells: a clue to the cell of origin and a marker for the diagnosis of dermatofibroma

VSports app下载 - Sang Yun Jin et al. Ann Dermatol. 2015 Apr.

Abstract (V体育官网)

Background: Dermatofibroma (DF) comprises a heterogeneous group of mesenchymal tumors, with fibroblastic and histiocytic elements present in varying proportions. The cell of origin of DF has been investigated, but remains unclear VSports手机版. .

Objective: The present study attempted to investigate the expression of leukocyte-specific protein 1 (LSP1), a marker of fibrocytes, in DF. Additionally, we evaluated the effectiveness of LSP1 in the differential diagnosis of DF from dermatofibrosarcoma protuberans (DFSP). V体育安卓版.

Methods: Immunohistochemical staining was performed on 20 cases of DF using antibodies against LSP1, CD68, and factor XIIIa (FXIIIa). In addition, the expression of LSP1 and FXIIIa was evaluated in 20 cases of DFSP. V体育ios版.

Results: Eighteen of 20 cases (90%) of DF stained positive for LSP1, with variation in the intensity of expression VSports最新版本. CD68 was positive in 10 cases (50%), and FXIIIa was expressed in all cases of DF. There were differences between the regional expression patterns of the three markers in individual tumors. In contrast, only 2 of 20 cases of DFSP expressed LSP1, and none of DFSP cases stained positive for FXIIIa. .

Conclusion: The LSP1-positive cells in DF could potentially be fibrocyte-like cells. FXIIIa and CD68 expression suggests that dermal dendritic cells and histiocytes are constituent cells of DF V体育平台登录. It is known that fibrocytes, dermal dendritic cells and histiocytes are all derived from CD14+ monocytes. Therefore, we suggest that DF may originate from CD14+ monocytes. Additionally, the LSP1 immunohistochemical stain could be useful in distinguishing between DF and DFSP. .

Keywords: Dermatofibroma; Dermatofibrosarcoma; Leukocyte-specific protein 1. VSports注册入口.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1. Histological sections of dermatofibroma stained with antibodies against (A, B) leukocyte-specific protein 1 (LSP1), (C, D) factor XIIIa (FXIIIa), and (E, F) CD68. Expression of LSP1 and FXIIIa was observed in both (A, C) cellular lesions and (B, D) fibrous lesions. In contrast, immunoreactivity of CD68 was stronger in (E) cellular lesions than (F) fibrous lesions (A~F: ×200).
Fig. 2
Fig. 2. Histological sections of dermatofibroma and normal skin stained with antibodies to (A, C) factor XIIIa (FXIIIa) and (B, D) leukocyte-specific protein 1 (LSP1). The expression patterns of (A) FXIIIa and (B) LSP1 were different in the same specimen of dermatofibroma. (C) FXIIIa-positive cells could be observed sporadically in the normal dermis, whereas (D) LSP1-positive cells could not be observed except for in a few lymphocytes in the normal dermis (A, B: ×40, C, D: ×100).
Fig. 3
Fig. 3. Histological sections of dermatofibrosarcoma protuberans stained with leukocyte-specific protein 1 (LSP1) antibody. LSP1 staining in the lesion was negative (×200).
See this image and copyright information in PMC

References (V体育ios版)

    1. Song Y, Sakamoto F, Ito M. Characterization of factor XIIIa+ dendritic cells in dermatofibroma: Immunohistochemical, electron and immunoelectron microscopical observations. J Dermatol Sci. 2005;39:89–96. - PubMed
    1. Horenstein MG, Prieto VG, Nuckols JD, Burchette JL, Shea CR. Indeterminate fibrohistiocytic lesions of the skin: is there a spectrum between dermatofibroma and dermatofibrosarcoma protuberans. Am J Surg Pathol. 2000;24:996–1003. - PubMed
    1. Yan X, Takahara M, Xie L, Tu Y, Furue M. Cathepsin K expression: a useful marker for the differential diagnosis of dermatofibroma and dermatofibrosarcoma protuberans. Histopathology. 2010;57:486–488. - PubMed
    1. Goldblum JR, Tuthill RJ. CD34 and factor-XIIIa immunoreactivity in dermatofibrosarcoma protuberans and dermatofibroma. Am J Dermatopathol. 1997;19:147–153. - PubMed
    1. Kuroda K, Tajima S. Proliferation of HSP47-positive skin fibroblasts in dermatofibroma. J Cutan Pathol. 2008;35:21–26. - PubMed

LinkOut - more resources