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Clinical Trial
. 2014 Oct 16;371(16):1507-17.
doi: 10.1056/NEJMoa1407222.

Chimeric antigen receptor T cells for sustained remissions in leukemia

Shannon L Maude  1 , Noelle Frey, Pamela A Shaw, Richard Aplenc, David M Barrett, Nancy J Bunin, Anne Chew, Vanessa E Gonzalez, Zhaohui Zheng, Simon F Lacey, Yolanda D Mahnke, Jan J Melenhorst, Susan R Rheingold, Angela Shen, David T Teachey, Bruce L Levine, Carl H June, David L Porter, Stephan A Grupp
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Clinical Trial

VSports最新版本 - Chimeric antigen receptor T cells for sustained remissions in leukemia

Shannon L Maude et al. N Engl J Med. .

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Abstract

Background: Relapsed acute lymphoblastic leukemia (ALL) is difficult to treat despite the availability of aggressive therapies. Chimeric antigen receptor-modified T cells targeting CD19 may overcome many limitations of conventional therapies and induce remission in patients with refractory disease VSports手机版. .

Methods: We infused autologous T cells transduced with a CD19-directed chimeric antigen receptor (CTL019) lentiviral vector in patients with relapsed or refractory ALL at doses of 0 V体育安卓版. 76×10(6) to 20. 6×10(6) CTL019 cells per kilogram of body weight. Patients were monitored for a response, toxic effects, and the expansion and persistence of circulating CTL019 T cells. .

Results: A total of 30 children and adults received CTL019. Complete remission was achieved in 27 patients (90%), including 2 patients with blinatumomab-refractory disease and 15 who had undergone stem-cell transplantation. CTL019 cells proliferated in vivo and were detectable in the blood, bone marrow, and cerebrospinal fluid of patients who had a response. Sustained remission was achieved with a 6-month event-free survival rate of 67% (95% confidence interval [CI], 51 to 88) and an overall survival rate of 78% (95% CI, 65 to 95) V体育ios版. At 6 months, the probability that a patient would have persistence of CTL019 was 68% (95% CI, 50 to 92) and the probability that a patient would have relapse-free B-cell aplasia was 73% (95% CI, 57 to 94). All the patients had the cytokine-release syndrome. Severe cytokine-release syndrome, which developed in 27% of the patients, was associated with a higher disease burden before infusion and was effectively treated with the anti-interleukin-6 receptor antibody tocilizumab. .

Conclusions: Chimeric antigen receptor-modified T-cell therapy against CD19 was effective in treating relapsed and refractory ALL VSports最新版本. CTL019 was associated with a high remission rate, even among patients for whom stem-cell transplantation had failed, and durable remissions up to 24 months were observed. (Funded by Novartis and others; CART19 ClinicalTrials. gov numbers, NCT01626495 and NCT01029366. ). .

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Figures

Figure 1
Figure 1. Probability of Event-free and Overall Survival at 6 Months
Panel A shows the time to an event after infusion of CTL019. Events were relapse (in seven patients), no response (in three patients), and the myelodysplastic syndrome (in one patient). Tick marks indicate the time of data censoring at the last follow-up or on the date of initiation of alternative therapy (in four patients). The curve in Panel B shows overall survival. Data were censored at the time of the last follow-up. In both panels, dashed lines represent 95% confidence intervals.
Figure 2
Figure 2. Persistence of CTL019
Panel A shows the results of detection of CTL019-positive T cells detected by means of flow cytometry in peripheral-blood samples. “Confirmed negative” was defined as the first of two consecutive negative measurements (<0.1% CTL019-positive cells in CD3-positive cells). Patients 1 through 25 were participants in the pediatric trial (which included children and young adults 5 to 22 years of age), and Patients 26 through 30 were participants in the adult trial (which included patients 26 to 60 years of age). CTL019-modified T cells were also detected in the cerebrospinal fluid of 17 of 19 patients with specimens that could be evaluated. Panel B shows the Kaplan–Meier curve of the time to the first confirmed negative measurement in peripheral blood and bone marrow. Data were censored at the time of the last follow-up. Dashed lines represent 95% confidence intervals. Panel C shows measurements of CTL019 gene-modified T cells in peripheral blood as assessed by means of quantitative real-time polymerase-chain-reaction (PCR) assay. Genomic DNA was isolated from samples of whole blood obtained at serial time points before and after infusion of CTL019. The horizontal line at 5 copies per microgram of DNA represents the lower limit of quantification of this assay. Data on patients who did not have a response are shown in red. In general, the levels of CTL019 detected by means of quantitative PCR correlated well with the level of CTL019-positive cells detected by means of flow cytometry, with the exception of the levels in 3 patients who did not have a response and whose peak levels measured by means of quantitative PCR (6066, 5982, and 178,481 copies per microgram of genomic DNA, respectively) did not correspond with detection of CTL019 cells by means of flow cytometry or the induction of B-cell aplasia. CTL019 sequences were detected (23 copies of CTL019 cells per microgram of DNA) at month 24 by means of quantitative PCR in the 1 patient who remained in complete remission at the 2-year follow-up. Data at the first time point were obtained before infusion of CTL019 cells. Doses of cells were determined according to the total amount of cells available after manufacturing. The manufacturing goal of 1.5×107 to 5×109 total cells (3×105 to 1×108 cells per kilogram of body weight) was achieved in all treated patients (see Table S2 in the Supplementary Appendix). A split-dose strategy was used to determine safety with 0.1×108 to 1×108 cells per kilogram infused over 1 to 3 days (5×108 to 50×108 cells in patients who weighed 50 kg or more). The transduction efficiency ranged from 5.5 to 45.3%; this yielded a dose of 0.76 to 20.6×106 CTL019 cells per kilogram.
Figure 3
Figure 3. B-Cell Aplasia
Panel A shows the results of testing to detect the percentage of CD19-positive lymphocytes in peripheral-blood samples by means of flow cytometry. Patients 1 through 25 were participants in the pediatric trial (which included children and young adults 5 to 22 years of age), and Patients 26 through 30 were participants in the adult trial (which included patients 26 to 60 years of age). Negative results were defined as less than 3% of lymphocytes that were positive for CD19. An outlier sample (Patient 16 at month 3) with 4% CD19-positive lymphocytes was discrepant with the measurements on clinical flow cytometry (<1% CD19-positive cells), bone marrow measurements at the same time point, and four subsequent monthly evaluations and was, therefore, considered to be negative. Panel B shows a Kaplan–Meier curve of the time to either CD19 positivity or relapse. Data on patients in remission were censored at the time of the last follow-up (indicated by tick marks). Dashed lines represent 95% confidence intervals. All patients required intravenous immunoglobulin replacement, and no serious infectious complications were observed as a result of B-cell aplasia; however, bronchitis (in one patient), acute otitis media (in two patients), salmonella infection (in one patient), and recurrent urinary tract infections (in one patient) were observed.
Figure 4
Figure 4. Correlates of the Cytokine-Release Syndrome
Panel A shows peak levels of interleukin-6 in the first 28 days after infusion of CTL019 cells in patients with severe cytokine-release syndrome as compared with patients with cytokine-release syndrome that was not severe. Severe cytokine-release syndrome was defined as hypotension requiring the use of two or more vasopressors or respiratory failure requiring mechanical ventilation. Panel B shows the severity of cytokine-release syndrome according to the baseline disease burden in bone marrow after chemotherapy and before infusion (in the pediatric trial only). Solid circles indicate complete remission, open circles no response, and horizontal lines medians.
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