Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 2014 Apr 14;9(4):e94863.
doi: 10.1371/journal.pone.0094863. eCollection 2014.

Faecal microbiota composition in adults is associated with the FUT2 gene determining the secretor status

Pirjo Wacklin  1 , Jarno Tuimala  1 , Janne Nikkilä  1 , Sebastian Tims  2 , Harri Mäkivuokko  1 , Noora Alakulppi  1 , Pia Laine  3 , Mirjana Rajilic-Stojanovic  2 , Lars Paulin  3 , Willem M de Vos  4 , Jaana Mättö  1
Affiliations

Faecal microbiota composition in adults is associated with the FUT2 gene determining the secretor status

Pirjo Wacklin et al. PLoS One. .

Abstract

The human intestine is colonised with highly diverse and individually defined microbiota, which likely has an impact on the host well-being VSports手机版. Drivers of the individual variation in the microbiota compositions are multifactorial and include environmental, host and dietary factors. We studied the impact of the host secretor status, encoded by fucosyltransferase 2 (FUT2) -gene, on the intestinal microbiota composition. Secretor status determines the expression of the ABH and Lewis histo-blood group antigens in the intestinal mucosa. The study population was comprised of 14 non-secretor (FUT2 rs601338 genotype AA) and 57 secretor (genotypes GG and AG) adult individuals of western European descent. Intestinal microbiota was analyzed by PCR-DGGE and for a subset of 12 non-secretor subjects and 12 secretor subjects additionally by the 16S rRNA gene pyrosequencing and the HITChip phylogenetic microarray analysis. All three methods showed distinct clustering of the intestinal microbiota and significant differences in abundances of several taxa representing dominant microbiota between the non-secretors and the secretors as well as between the FUT2 genotypes. In addition, the non-secretors had lower species richness than the secretors. The soft clustering of microbiota into enterotypes (ET) 1 and 3 showed that the non-secretors had a higher probability of belonging to ET1 and the secretors to ET3. Our study shows that secretor status and FUT2 polymorphism are associated with the composition of human intestinal microbiota, and appears thus to be one of the key drivers affecting the individual variation of human intestinal microbiota. .

PubMed Disclaimer

Conflict of interest statement

Competing Interests: For conflict of interests, the authors declare that PW, HM and JM are inventors in patent applications (20110158950, Use of Blood group status I; 20110158951, Use of Blood group status II; 20120020941 Use of Blood group status III). Of these, patent application 20120020941 “Use of Blood group status III” is related to the data used in the manuscript V体育安卓版. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.

"VSports手机版" Figures

Figure 1
Figure 1. RDA plots of dominant microbiota composition in the secretors (black) and the non-secretors (white) (A) and the FUT2 genotypes, AA (white), AG (grey) and GG (black) (B).
The plots are based on the PCR-DGGE analysis with universal primers. The centroids of each group are indicated by triangles. P-values show statistical significance in ANOVA test. The RDA plots of DGGE analysis with the specific bacterial groups are shown in Figures S1 and S2.
Figure 2
Figure 2. RDA plots of microbiota composition in the non-secretors (white) and the secretors (black) (A) and among FUT2 genotypes, AA (white), AG (grey) and GG (black) (B).
Plots are calculated from the relative abundances of taxa obtained by the 16S rRNA gene pyrosequencing. The centroids of each group are indicated by triangles. P-values show statistical significance in ANOVA test.
Figure 3
Figure 3. Mean relative abundances of the bacterial genera showing significant (ANOVA/indicator species analysis, p<0.05) or trend-like differences (p<0.09) between the non-secretors (n = 12) and the secretors (n = 12) or between the FUT2 genotypes AA (n = 12), AG (n = 7), GG (n = 5).
The statistical significance is indicated for a comparison of the secretors/FUT2 genotypes AG and GG to the non-secretors/FUT2 genotype AA. The taxa significantly differing in both indicator species analysis and ANOVA test are underlined.
Figure 4
Figure 4. The RDA plots of microbiota composition in the non-secretors (white) and the secretors (black) (A) and among the individuals with FUT2 genotypes, AA (white), AG (grey) and GG (black) (B).
The RDA plots were based on genus-like/level 2 intensities of HITChip analysis. The centroids of each group are indicated by triangles. P-values show statistical significance in ANOVA test.
See this image and copyright information in PMC

References

    1. Rajilic-Stojanovic M, Heilig HG, Tims S, Zoetendal EG, de Vos WM (2013) Long-term monitoring of the human intestinal microbiota composition. Environ Microbiol 15: 1146–1159. - "VSports" PubMed
    1. Zoetendal EG, Akkermans ADL, Akkermans-van Vliet WM, de Visser, J. Arjan G M, et al. (2001) The Host Genotype Affects the Bacterial Community in the Human Gastronintestinal Tract. Microbial Ecology in Health and Disease 13: 129–134.
    1. Tims S, Derom C, Jonkers DM, Vlietinck R, Saris WH, et al. (2013) Microbiota conservation and BMI signatures in adult monozygotic twins. ISME J 7: 707–717. - PMC - PubMed
    1. Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, et al. (2009) A core gut microbiome in obese and lean twins. Nature 457: 480–484. - PMC - PubMed
    1. Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, et al. (2012) Human gut microbiome viewed across age and geography. Nature 486: 222–227. - PMC - PubMed

"VSports" Publication types

V体育官网入口 - Substances

LinkOut - more resources (VSports app下载)