"VSports app下载" Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation

The microbiome of translocated bacterial populations in patients with and without inflammatory bowel disease (VSports)

Kiely1, Pavli2, O’Brien3 2018Internal Medicine Journal201150Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…mRNA undergoes rapid metabolism by intracellular and extracellular RNase 15 and has an overall lifetime of only a few minutes to hours. 26,27 The aims of our study were: to investigate the existence of viable organisms in the lymph nodes of subjects with and without IBD using molecular techniques and to compare the bacteria present in the lymph nodes with the same patient's gut microbiome. 17 While 16S rRNA is generally stable, degradation occurs under stressful conditions. …”Section: Introductionmentioningconfidence: 99%“…However, as this has been associated with a moderate false positive rate, 25 RNA detection is preferred in tissues with low bacterial abundances. 26,27 The aims of our study were: to investigate the existence of viable organisms in the lymph nodes of subjects with and without IBD using molecular techniques and to compare the bacteria present in the lymph nodes with the same patient's gut microbiome. …”Section: Introductionmentioningconfidence: 99%

The microbiome of translocated bacterial populations in patients with and without inflammatory bowel disease

Kiely1, Pavli2, O’Brien3 2018Internal Medicine Journal201150Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…One obvious inconsistency is the use of propidium monoazide (PMA), a chemical compound that binds DNA in cells with damaged membranes and hence allows exclusion of non-viable DNA from sequencing. Excluding non-viable DNA has been suggested to be important for accurately identifying which members of the community are active at times of exacerbation and helps to avoid overestimation of viable microorganisms following treatment with antibiotics, but it is not utilised by all groups [51,52]. There are concerns that PMA may incompletely penetrate sputum, hence only identifying a portion of non-viable cells. …”Section: Community Changes Associated With Treatment For Acute Pulmonmentioningconfidence: 99%

The Pulmonary Microbiome in Cystic Fibrosis

Frost1, Nazareth2, Wat3 2020Cystic Fibrosis - Heterogeneity and Personalized Treatment0000Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…Therefore, it is vital that such analyses choose an appropriate and consistent DNA extraction method which will provide efficient extraction from all cell types and comparable extraction efficiency from different sample types. Nyugen et al performed 16S and internal transcribed spacer (ITS) sequencing and identified Candida as the most predominant fungal genera in CF sputum samples [67]. However, the enzyme-based DNA extraction protocol they used may have favoured the extraction of yeast compared to fungi. …”Section: Limiting Factors Of Sample Processing In Mycobiome Studiesmentioningconfidence: 99%“…A high person-to-person variation in mycobiome composition has been reported. Few studies to date have analysed samples in replicates and most only include technical replicates at the PCR stage [37,67]. Bittinger et al [20] performed repeat extractions from samples and found poor reproducibility between replicates, with instances of high abundance of an operational taxonomic unit (OTU) in one replicate and absence in another. …”Section: Reproducibility In Mycobiome Researchmentioningconfidence: 99%

VSports在线直播 - The Human Lung Mycobiome in Chronic Respiratory Disease: Limitations of Methods and Our Current Understanding

Weaver1, Gago2, Bromley3 et al. 2019Curr Fungal Infect Rep300390Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD) VSports app下载. Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstractScite is an AI-powered research tool that helps researchers better discover and evaluate scientific literature through Smart Citations—a revolutionary system that shows whether articles support, contrast, or simply mention a given claim. Founded in 2018, and now part of Research Solutions, Scite has indexed over 1. 4 billion citations and partnered with more than 30 major publishers to provide researchers with unparalleled access to scientific literature. With its Scite Assistant, Smart Citation Index, and advanced search capabilities, the platform addresses critical challenges such as information overload and research reproducibility. Trusted by two million active users worldwide, Scite is reshaping how researchers interact with scholarly content—building ethical, transparent AI tools that support rigorous, copyright-compliant research.