Low-density granulocytes: functionally distinct, immature neutrophils in rheumatoid arthritis with altered properties and defective TNF signalling

"V体育2025版" Human neutrophils activated via TLR8 promote Th17 polarization through IL-23

Tamassia1, Arruda-Silva2, Wright3 et al. 2019Journal of Leukocyte BiologySelf Cite455420Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…Highly purified neutrophils were isolated from the venous blood or from buffy coats from healthy individuals using a combined method consisting of Ficoll‐Paque gradient centrifugation, dextran or Hetasep (StemCell Technologies, Vancouver, Canada) sedimentation of granulocytes, and hypotonic lysis of erythrocytes, followed by removal of contaminating immune cells using the EasySep neutrophil enrichment kit (StemCell Technologies, Vancouver, Canada) as described previously . This procedure yields neutrophils of ∼99. 7% purity. …”Section: Methodsmentioningconfidence: 99%

Human neutrophils activated via TLR8 promote Th17 polarization through IL-23

Tamassia1, Arruda-Silva2, Wright3 et al. 2019Journal of Leukocyte BiologySelf Cite455420Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…Humans Most of our knowledge of neutrophil heterogeneity in chronic inflammation derives from studies performed in autoimmune diseases [54,96]. In these studies, the presence of LDNs has been consistently reported in patients with systemic lupus erythematosus (SLE), psoriasis, chronic granulomatous disease, ANCA-associated systemic vasculitis, rheumatoid arthritis (RA), juvenile idiopathic arthritis, and pyogenic arthritis-pyoderma gangrenosum-acne syndrome [54,[96][97][98][99][100]. As opposed to immunosuppressive LDNs/PMN-MDSCs, LDNs from autoimmune patients have been defined as low-density granulocytes (LDGs) (Box 1 and Figure 3) based on their proinflammatory properties [4,7,54,96], including the promotion of Th17 cell differentiation and proliferation [101]. …”Section: Neutrophil Diversity and Heterogeneity In Chronic Inflammationmentioningconfidence: 99%“…As opposed to immunosuppressive LDNs/PMN-MDSCs, LDNs from autoimmune patients have been defined as low-density granulocytes (LDGs) (Box 1 and Figure 3) based on their proinflammatory properties [4,7,54,96], including the promotion of Th17 cell differentiation and proliferation [101]. It is important to note that the distinction between LDNs/LDGs and LDNs/PMN-MDSCs is based only on functional assays performed on isolated cells because no validated markers have been identified that can discriminate between suppressive and proinflam- or 'low-density granulocytes' (LDGs) (Box 1) have been found in patients with autoimmune diseases, including systemic lupus erythematosus (SLE), psoriasis, chronic granulomatous disease, anti-neutrophil cytoplasmic autoantibody (ANCA)associated vasculitis, rheumatoid arthritis (RA), juvenile idiopathic arthritis, and pyogenic arthritis-pyoderma gangrenosumacne syndrome [96][97][98][99][100]. However, not all studies tested/reported the proinflammatory properties of LDNs from autoimmune patients. …”Section: Neutrophil Diversity and Heterogeneity In Chronic Inflammationmentioningconfidence: 99%

Neutrophil Diversity in Health and Disease

Silvestre-Roig1, Fridlender2, Glogauer3 et al. 2019Trends in Immunology46074221Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. “…Low-density granulocytes (LDG) have been previously described in several pathological conditions, such as systemic lupus erythematosus, rheumatoid arthritis, asthma, and HIV infection, where elevated LDG levels correlated with disease severity [108–111]. A characteristic feature of LDG is their remaining in the PBMC layer after density-gradient centrifugation along with the expression of the main neutrophil markers (i VSports app下载. e. , CD15, CD66b, and CD11b) and lack of the expression of CD14. …”Section: Neutrophils During Tb: Are They the Same Cells As In Steamentioningconfidence: 99%

"VSports在线直播" Neutrophils in Tuberculosis: Heterogeneity Shapes the Way?

Lyadova1 2017Mediators of Inflammation10041070Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstractScite is an AI-powered research tool that helps researchers better discover and evaluate scientific literature through Smart Citations—a revolutionary system that shows whether articles support, contrast, or simply mention a given claim. Founded in 2018, and now part of Research Solutions, Scite has indexed over 1. 4 billion citations and partnered with more than 30 major publishers to provide researchers with unparalleled access to scientific literature. With its Scite Assistant, Smart Citation Index, and advanced search capabilities, the platform addresses critical challenges such as information overload and research reproducibility. Trusted by two million active users worldwide, Scite is reshaping how researchers interact with scholarly content—building ethical, transparent AI tools that support rigorous, copyright-compliant research.