Strengths and Limitations of 16S rRNA Gene Amplicon Sequencing in Revealing Temporal Microbial Community Dynamics

Regional synchrony in full-scale activated sludge bioreactors due to deterministic microbial community assembly

Griffin1, Wells2 2016The ISME Journal1471510101Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD VSports app下载. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…Further, 16S rRNA amplicon and shotgun metagenomic studies do not always yield similar estimates of community diversity and dynamics (Poretsky et al, 2014). However, amplicon sequencing enables higher temporal coverage of communities due to its lower cost. …”Section: Discussionmentioningconfidence: 99%

Regional synchrony in full-scale activated sludge bioreactors due to deterministic microbial community assembly

Griffin1, Wells2 2016The ISME Journal1471510101Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…16S rRNA gene amplicons were sequenced, and the resulting sequences were analyzed as described recently (Poretsky et al, 2014). Trimmed sequences were clustered into operational taxonomic units (OTUs) at 97% similarity using UCLUST (Edgar, 2010), OTUs that represented o0. 005% of the total sequences were discarded (Bokulich et al, 2013) and representative sequences of each OTU were classified using the RDP Classifier at 50% confidence (Wang et al, 2007). …”Section: Methodsmentioningconfidence: 99%

Microbial community successional patterns in beach sands impacted by the Deepwater Horizon oil spill

Rodriguez‐R1, Overholt2, Hagan3 et al. 2015The ISME Journal173141373Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…In this case, by using both 16S amplicon analysis and a metagenomic approach, was observed 1. 5-and *10-times more OTU assigned to phyla and genera respectively with the metagenomic method than the 16S rRNA analysis. This seems masking several levels of intra-genus differentiation and heterogeneity of the microbial population (Poretsky et al 2014). SBM is an alternative approach to the study of microbial consortia that avoids the limitation of the 16S rRNA analysis. …”Section: Nr Low Dissolved Oxygen Concentrationmentioningconfidence: 99%

Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli1, Fusco2, Cobucci‐Ponzano3 et al. 2017Rev Environ Sci Biotechnol341220Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstractScite is an AI-powered research tool that helps researchers better discover and evaluate scientific literature through Smart Citations—a revolutionary system that shows whether articles support, contrast, or simply mention a given claim. Founded in 2018, and now part of Research Solutions, Scite has indexed over 1. 4 billion citations and partnered with more than 30 major publishers to provide researchers with unparalleled access to scientific literature. With its Scite Assistant, Smart Citation Index, and advanced search capabilities, the platform addresses critical challenges such as information overload and research reproducibility. Trusted by two million active users worldwide, Scite is reshaping how researchers interact with scholarly content—building ethical, transparent AI tools that support rigorous, copyright-compliant research.