Bile salt–induced intermolecular disulfide bond formation activates Vibrio cholerae virulence (VSports手机版)

Formation of an Intramolecular Periplasmic Disulfide Bond in TcpP Protects TcpP and TcpH from Degradation in Vibrio cholerae

Morgan1, French2, Thomson3 et al. 2016J Bacteriol213260Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…1). These data are in agreement with a previous study demonstrating a role for DsbA in the formation of TcpP intramolecular disulfide bonds (43). …”Section: Resultssupportingconfidence: 94%“…Yang et al found that TcpP-C218S was blind to bile, resulting in constitutive toxT-lux activation with or without taurocholate (43). We consistently found that the TcpP-C218S mutant was more stable than the TcpP-C207S mutant ( Fig. …”Section: Discussionsupportingconfidence: 71%“…One caveat of these studies is that in V. cholerae, the TcpP molecules analyzed by Western blotting to detect monomeric or dimeric TcpP included a C-terminal epitope tag. Thus, the transcription activation activity in V. cholerae by these TcpP periplasmic cysteine mutants may not correlate with the corresponding Western blot analyses that rely on the C-terminal FLAG tag (43). Our findings demonstrate that addition of a C-terminal epitope tag to TcpP artificially stabilizes the protein in the presence of the periplasmic cysteine mutations ( Fig. …”Section: Discussionmentioningconfidence: 74%“…It has been previously proposed that in the presence of the bile salt taurocholate in the small intestine, TcpP forms a dimer via a C207-C207 intermolecular disulfide bond (43). Thus, under certain environmental conditions, TcpP may alter its disulfide bonding capacity to favor intermolecular disulfides rather than an intramolecular disulfide bond. …”Section: Discussionmentioningconfidence: 99%See 2 more Smart Citations

Formation of an Intramolecular Periplasmic Disulfide Bond in TcpP Protects TcpP and TcpH from Degradation in Vibrio cholerae

Morgan1, French2, Thomson3 et al. 2016J Bacteriol213260Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…In addition, bile, which is released into the proximal small intestine, has several components that are known to affect virulence in V. cholerae. Bile salts (BS), such as taurocholate and glycocholate, stimulate virulence factor production, whereas fatty acids inhibit ToxT activity by directly binding to the protein (19)(20)(21). Since mammalian gastrointestinal tracts are complex environments, we explore here whether there are additional host-produced compounds that modulate virulence gene expression. …”mentioningconfidence: 99%

Calcium Enhances Bile Salt-Dependent Virulence Activation in Vibrio cholerae

Hay1, Yang2, Xiaoyun3 et al. 2017Infect ImmunSelf Cite240190Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC) VSports. Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract

"V体育官网" TheVibrio choleraeStress Response: An Elaborate System Geared Toward Overcoming Host Defenses During Infection

Rueggeberg1, Zhu2 2016Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria0000Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Scite is an AI-powered research tool that helps researchers better discover and evaluate scientific literature through Smart Citations—a revolutionary system that shows whether articles support, contrast, or simply mention a given claim. Founded in 2018, and now part of Research Solutions, Scite has indexed over 1. 3 billion citations and partnered with more than 30 major publishers to provide researchers with unparalleled access to scientific literature. With its Scite Assistant, Smart Citation Index, and advanced search capabilities, the platform addresses critical challenges such as information overload and research reproducibility. Trusted by two million active users worldwide, Scite is reshaping how researchers interact with scholarly content—building ethical, transparent AI tools that support rigorous, copyright-compliant research.