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. 1996 Jun:142 ( Pt 6):1537-1541.
doi: 10.1099/13500872-142-6-1537.

Collagen-binding activity of Prevotella intermedia measured by a microtitre plate adherence assay

Daniel Grenier  1
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VSports注册入口 - Collagen-binding activity of Prevotella intermedia measured by a microtitre plate adherence assay

Daniel Grenier. Microbiology (Reading). 1996 Jun.
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V体育ios版 - Abstract

The ability of Prevotella intermedia to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity VSports手机版. All strains of P. intermedia tested, as well as those of the closely related species Prevotella nigrescens, showed a capacity to attach to the collagen film. Exponential-phase cultures of P. intermedia demonstrated a greater binding capacity than older cells. Attachment to the collagen film was inhibited by the presence of EDTA, type I and IV collagen, denatured collagen (gelatin), fibrinogen or fibronectin. Pretreatment of bacterial cells with heat (60 degrees C, 30 min) or proteinase K also inhibited the binding. The collagen-binding activity could be solubilized from the bacterial cell surface by incubation with Zwittergent 3-14, a zwitterionic detergent. The collagen-binding capacity of P. intermedia demonstrated in the present study represents a mechanism of colonization allowing these bacteria to attach to a tissue matrix. .

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