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Comparative Study
. 1995 Aug;177(15):4392-401.
doi: 10.1128/jb.177.15.4392-4401.1995.

Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii

R Daniel  1 , K Stuertz, G Gottschalk
Affiliations
Comparative Study

"V体育ios版" Biochemical and molecular characterization of the oxidative branch of glycerol utilization by Citrobacter freundii

R Daniel et al. J Bacteriol. 1995 Aug.

Abstract

Glycerol dehydrogenase (EC 1. 1. 1. 6) and dihydroxyacetone kinase (EC 2. 7. 1. 29) were purified from Citrobacter freundii. The dehydrogenase is a hexamer of a polypeptide of 43,000 Da. The enzyme exhibited a rather broad substrate specificity, but glycerol was the preferred substrate in the physiological direction VSports手机版. The apparent Kms of the enzyme for glycerol and NAD+ were 1. 27 mM and 57 microM, respectively. The kinase is a dimer of a polypeptide of 57,000 Da. The enzyme was highly specific for the substrates dihydroxyacetone and ATP; the apparent Kms were 30 and 70 microM, respectively. The DNA region which contained the genes encoding glycerol dehydrogenase (dhaD) and dihydroxyacetone kinase (dhaK) was cloned and sequenced. Both genes were identified by N-terminal sequence comparison. The deduced dhaD gene product (365 amino acids) exhibited high degrees of homology to glycerol dehydrogenases from other organisms and less homology to type III alcohol dehydrogenases, whereas the dhaK gene product (552 amino acids) revealed no significant homology to any other protein in the databases. A large gene (dhaR) of 1,929 bp was found downstream from dhaD. The deduced gene product (641 amino acids) showed significant similarities to members of the sigma 54 bacterial enhancer-binding protein family. .

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References

    1. J Bacteriol. 1992 Jul;174(13):4391-400 - PubMed
    1. Gene. 1988 Jun 30;66(2):301-6 - PubMed
    1. J Biol Chem. 1992 Sep 5;267(25):18073-9 - PubMed (VSports注册入口)
    1. J Bacteriol. 1992 Nov;174(22):7149-58 - PubMed (V体育ios版)
    1. J Mol Biol. 1988 Oct 5;203(3):715-38 - PubMed

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