Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official VSports app下载. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 1995 Nov;63(11):4476-80.
doi: 10.1128/iai.63.11.4476-4480.1995.

Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies

R Manetti  1 , P Massari, D Burroni, M de Bernard, A Marchini, R Olivieri, E Papini, C Montecucco, R Rappuoli, J L Telford
Affiliations

VSports - Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies

R Manetti et al. Infect Immun. 1995 Nov.

Abstract

We have attempted to express the Helicobacter pylori vacuolating cytotoxin in Escherichia coli. Although the 95-kDa VacA polypeptide was expressed abundantly, it completely lacked any biological activity. In addition, this material failed to induce neutralizing antibodies after immunization of rabbits. In contrast, highly purified high-molecular-mass cytotoxin from the supernatant of H. pylori cultures was active in a HeLa cell assay and effectively induced a neutralizing response in rabbits. Neutralizing sera were shown to contain a high proportion of antibodies which recognized conformational epitopes found only on the native toxin. The data indicate that toxin-neutralizing epitopes are conformational and that potential vaccines based on the cytotoxin may benefit from the use of the intact molecule. VSports手机版.

PubMed Disclaimer

References

    1. J Biol Chem. 1994 Apr 8;269(14):10566-73 - VSports在线直播 - PubMed
    1. Science. 1995 Mar 17;267(5204):1655-8 - "VSports最新版本" PubMed
    1. J Exp Med. 1994 May 1;179(5):1653-58 - PubMed
    1. Mol Microbiol. 1994 Apr;12(2):307-19 - PubMed
    1. Structure. 1994 Jan 15;2(1):45-57 - PubMed

MeSH terms

LinkOut - more resources (V体育安卓版)