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. 2022 Nov;14(11):4246-4255.
doi: 10.21037/jtd-22-324.

The effects of hydrogen treatment in a cigarette smoke solution-induced chronic obstructive pulmonary disease-like changes in an animal model

Hui-Ju Yang  1 , Wen-Hsin Tsou  1 , Min-Chung Shen  2 , Chian-Yi Liu  3 , Hsiu-Ming Saunders  1   4 , Kuang-Yih Wang  1 , Frank Lennox Douglas  1
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"V体育官网入口" The effects of hydrogen treatment in a cigarette smoke solution-induced chronic obstructive pulmonary disease-like changes in an animal model

Hui-Ju Yang et al. J Thorac Dis. 2022 Nov.

Erratum in

Abstract

Background: Molecular hydrogen, with its antioxidant and anti-inflammatory properties, may be suitable for the prevention and treatment of chronic obstructive pulmonary disease (COPD) VSports手机版. This study aims to investigate the therapeutic efficacy of hydrogen-oxygen (H2/O2) treatment in cigarette smoke solution (CSS)-induced COPD-like injury in a female BALB/c mouse model. .

Methods: Thirty mice were randomly assigned to three groups: Control (n=8), COPD (n=10), and COPD + H2/O2 (n=12). CSS was administered by intraperitoneal (IP) injection twice weekly for 6 weeks during the COPD induction phase. Simultaneously, the COPD + H2/O2 group started received 75 minutes of inhalation therapy (42% H2) delivered by the Oxy-Hydrogen Generator twice daily for 9 weeks V体育安卓版. Mice body weights and survival were measured throughout the study period. Neutrophil elastase (NE) activity and lung histopathological changes were also evaluated. .

Results: The results showed a higher survival rate in the COPD + H2/O2 group compared to the COPD group (100% vs V体育ios版. 80%) during the induction phase. Slight decreases in body weight gains were observed in the COPD and COPD + H2/O2 groups during the first 15 days of the induction phase, but there was no significant difference in mean body weights among the three groups throughout the study period. NE activity was numerically lower in the COPD + H2/O2 group compared to the COPD group. The histopathological evaluation showed significant improvements in the H2/O2-treated mice with respect to mean linear intercept (MLI) and lesion (inflammation and emphysema) scores. Improvements in goblet cell hypertrophy and hyperplasia of airway epithelium were not significant. .

Conclusions: A 9-week H2/O2 inhalation therapy delivered by the Oxy-Hydrogen Generator to CSS-induced COPD-like injury in mice showed improvement in survival rate, alveolar structural changes, and histopathological lesion scores of the lung. VSports最新版本.

Keywords: Hydrogen gas; chronic obstructive pulmonary disease (COPD); inflammation; oxidative stress. V体育平台登录.

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Conflict of interest statement

Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://jtd. amegroups. com/article/view/10. 21037/jtd-22-324/coif). HJY, WHT, HMS, KYW, and FLD reports funding from HOHO Biotech Co. , Ltd. HJY is the medical advisor of HOHO Biotech. KYW is the founder of HOHO Biotech. WHT is the employee of HOHO Biotech V体育官网入口. HMS and FLD are board members of HOHO Biotech. HMS is the person in charge for IPC Intellectual Property Connections, INC. She declares that IPC Intellectual Property Connections, INC. has no relationship with HOHO and does not have any interest in the publication of the present article. The other authors have no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
The protocol for the hydrogen gas inhalation experiment in the CSS-induced COPD-injury mouse model. NE activity in each group was pre-analyzed by using fluorescence X-ray before sacrifice. CSS, cigarette smoke solution; IP injection, intraperitoneal injection; COPD, chronic obstructive pulmonary disease. H2/O2, hydrogen-oxygen; NE, neutrophil elastase.
Figure 2
Figure 2
Effect of H2/O2 treatment on survival rates. COPD, chronic obstructive pulmonary disease; H2/O2, hydrogen-oxygen; CSS, cigarette smoke solution.
Figure 3
Figure 3
Changes in body weight, expressed as the percentage of initial body weight, during the study period. COPD, chronic obstructive pulmonary disease; H2/O2, hydrogen-oxygen; CSS, cigarette smoke solution.
Figure 4
Figure 4
The effects of COPD-like injury and H2/O2 treatment on NE activity. The area of pulmonary fluorescence images of NE activity was detected by in vivo X-treme imaging system. (A) Representative fluorescence images of NE activity. (B) A dot plot is showing the fluorescence intensity of animals in each group (n=6 for control and COPD group, and n=12 for COPD + H2/O2 group). COPD, chronic obstructive pulmonary disease; H2/O2, hydrogen-oxygen; NE, neutrophil elastase.
Figure 5
Figure 5
The effect of H2/O2 treatment on the histopathologic lung changes in CSS-induced COPD-like injury in mice. (A) Representative microphotographs showing histochemical staining of mouse lung tissue sections (H&E, ×200). The arrows indicate the alveolar wall destruction and airspace enlargement. (B) A dot plot is showing the MLI of animals in each group. **, P<0.05 as compared to the control; ##, P<0.05 as compared to the COPD group. n=4 for control and COPD group, n=6 for COPD + H2/O2 group. COPD, chronic obstructive pulmonary disease; H2/O2, hydrogen-oxygen; CSS, cigarette smoke solution; H&E, hematoxylin and eosin stain; MLI, mean linear intercept.
Figure 6
Figure 6
The effect of H2/O2 treatment on the histopathological changes in CSS-induced COPD-like injury in mice. (A) Representative microphotographs showing histochemical staining of mouse lung tissue sections (PAS, ×200). The arrows indicate goblet cell hyperplasia. (B) A dot plot is showing the PAS-positive area score of animals in each group. **, P<0.01. n=4 for control and COPD group, n=6 for COPD + H2/O2 group. COPD, chronic obstructive pulmonary disease; H2/O2, hydrogen-oxygen; PAS, Periodic Acid-Schiff stain; CSS, cigarette smoke solution.
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