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. 2022 Dec 6;19(1):25.
doi: 10.1186/s12950-022-00322-x.

"V体育2025版" Evaluation of asthma-chronic obstructive pulmonary disease overlap using a mouse model of pulmonary disease

Yong Suk Jo  1 , Chin Kook Rhee  1 , Hyoung Kyu Yoon  2 , Chan Kwon Park  2 , Jeong Uk Lim  2 , Tai Joon An  2 , Jung Hur  3
Affiliations

Evaluation of asthma-chronic obstructive pulmonary disease overlap using a mouse model of pulmonary disease

Yong Suk Jo (V体育安卓版) et al. J Inflamm (Lond). .

"V体育官网入口" Erratum in

Abstract

Background: Features of asthma and chronic obstructive pulmonary disease (COPD) can coexist in the same patient, in a condition termed asthma- chronic obstructive pulmonary disease overlap (ACO). ACO is heterogeneous condition exhibiting various combinations of asthma and COPD features. No clinically acceptable experimental model of ACO has been established. We aimed to establish an animal model of ACO. VSports手机版.

Methods: We generated two phenotypes of ACO by administering ovalbumin and porcine pancreatic elastase in combination, and papain. The proinflammatory cytokines and cell types in bronchoalveolar lavage fluid (BALF) were investigated, and lung function parameters were measured using the FlexiVent system V体育安卓版. .

Results: Greater airway inflammation was observed in the asthma and both ACO models, and emphysema was found in the COPD and both ACO models V体育ios版. The proportion of eosinophils in BALF was elevated in the asthma and ACO-a model. Type 2 inflammatory cytokine levels were highest in the ACO-a model, and the neutrophil gelatinase-associated lipocalin level was elevated in the asthma and ACO-a model. Of lung function parameters, compliance was greater in the COPD and ACO-b model, in which elastance was lower than in the asthma model. Airway resistance increased with the methacholine concentration in the asthma and both ACO models, but not in the control or COPD model. .

Conclusion: We established two murine models of ACO that exhibit features of asthma and COPD. We validated the clinical relevance of the ACO models based on changes in cytokine profiles and lung function VSports最新版本. These models will be useful in further studies of the pathogenesis of, and therapeutic targets for ACO. .

Keywords: Asthma; Chronic obstructive pulmonary disease; Cytokine; Experimental model; Lung function. V体育平台登录.

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Conflict of interest statement (V体育官网)

The authors have no potential conflict of interest relevant to this article.

Figures

Fig. 1
Fig. 1
Pulmonary disease models. ACO, asthma–chronic obstructive pulmonary disease overlap; alum, aluminum hydroxide; i.p., intraperitoneal; i.t., intratracheal; OVA, ovalbumin; PPE, porcine pancreatic elastase
Fig. 2
Fig. 2
Airway inflammation. A Differential cell counts in bronchoalveolar lavage fluid (BALF), B serum total IgE concentrations, C lung tissue stained with hematoxylin and eosin (20×), D inflammation scores. ***p < 0.001 vs. control
Fig. 3
Fig. 3
Inflammatory cytokines. A Type-2 inflammatory cytokines in BALF, B macrophage/neutrophil-associated inflammatory cytokines in BALF and serum, C NGAL in BALF and serum. *vs. control, #vs. asthma; *p < 0.05, **p < 0.01, ***p < 0.001
Fig. 4
Fig. 4
Respiratory mechanics. (A) Snapshot perturbation; resistance (R), elastance (E), and compliance (C). As the lung is seen as a single compartment, the parameters (R, E, and C) are indicative of the whole thorax (chest wall and lung). (B) Primewave-7 perturbation; airway resistance (Rn), tissue damping or resistance (G), and tissue elasticity (H). As the lung is seen as multiple compartments, Rn, G, and H can differentiate airways. (C) Airway responsiveness to increasing doses of methacholine measured by airway resistance. *vs. control, #vs. asthma; *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant
Fig. 5
Fig. 5
Lung tissue (A) from the airway models stained with hematoxylin and eosin (10×) to evaluate air space widening (emphysema). (B) Mean linear intercepts, calculated by measuring alveolar space diameters in random fields per slide using a slide scanner (Panoramic MIDI)
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References

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    1. Global Initiative for Chronic Obstructive Lung Disease. Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease: 2022 Update. Available online: http://www.goldcopd.org (accessed on 15 May 2022). [Internet].
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