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. 2021 Oct 28;31(10):1383-1392.
doi: 10.4014/jmb.2103.03014.

Efficacy of VSports app下载 - Lactobacillus fermentum Isolated from the Vagina of a Healthy Woman against Carbapenem-Resistant Klebsiella Infections In Vivo

Hanieh Tajdozian  1   2 , Hoonhee Seo  2 , Sukyung Kim  2 , Md Abdur Rahim  1   2 , Saebim Lee  2 , Ho-Yeon Song  1   2
Affiliations

Efficacy of Lactobacillus fermentum Isolated from the Vagina of a Healthy Woman against Carbapenem-Resistant Klebsiella Infections In Vivo

Hanieh Tajdozian et al. J Microbiol Biotechnol. .

Abstract

Carbapenem-resistant Enterobacteriaceae (CRE) that produce Klebsiella pneumoniae carbapenemase are increasingly reported worldwide and have become more and more resistant to nearly all antibiotics during the past decade. The emergence of K. pneumoniae strains with decreased susceptibility to carbapenems, which are used as a last resort treatment option, is a significant threat to hospitalized patients worldwide as K. pneumoniae infection is responsible for a high mortality rate in the elderly and immunodeficient individuals. This study used Lactobacillus fermentum as a candidate probiotic for treating CRE-related infections and investigated its effectiveness. We treated mice with L. fermentum originating from the vaginal fluid of a healthy Korean woman and evaluated the Lactobacilli's efficacy in preventive, treatment, non-establishment, and colonization mouse model experiments. Compared to the control, pre-treatment with L. fermentum significantly reduced body weight loss in the mouse models, and all mice survived until the end of the study. The oral administration of L. fermentum after carbapenemresistant Klebsiella (CRK) infection decreased mortality and illness severity during a 2-week observation period and showed that it affects other strains of CRK bacteria. Also, the number of Klebsiella bacteria was decreased to below 5. 5 log10 CFU/ml following oral administration of L VSports手机版. fermentum in the colonization model. These findings demonstrate L. fermentum's antibacterial activity and its potential to treat CRE infection in the future. .

Keywords: Carbapenem-resistant Enterobacteriaceae; Lactobacillus fermentum; carbapenem-resistant Klebsiella; mortality; mouse model. V体育安卓版.

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Conflict of interest statement

Conflict of Interest

The authors have no financial conflicts of interest to declare.

Figures

Fig. 1
Fig. 1. Results of acute toxicity assay of probiotics.
Nine-week-old BALB/c mice were treated with L. fermentum twice a week. Weights of mice were recorded for 2 weeks.
Fig. 2
Fig. 2. Prophylactic effect of probiotics in a CRK-infected mouse model.
(A) L. fermentum was administered 6 days before infection to confirm the preventive effect of probiotics. (B) The survival rate of the mice was observed for 2 weeks postinfection. (C) Illness severity score was evaluated during 8 days. (1, healthy; 2, minimally ill; 3, moderately ill; 4, severely ill; 5, dead). (D) Body weight was measured for 7 days. (E) Stool samples were collected from individual infected mice, and a CFU test for CRK was performed. Statistical significance with controls was analyzed using unpaired Student's t-test (***p < 0.001; **p < 0.01; *p < 0.05).
Fig. 3
Fig. 3. Therapeutic effect of probiotics on CRK in an established infectious mouse model.
(A) In order to evaluate the therapeutic effect of probiotics on CRK infection, probiotics treatment was started one day after clinical signs appeared after infection. (B) Survival rates, (C) Illness severity score, and (D) weight changes were observed or analyzed during probiotic treatment after infection. (E) Stool samples were collected from individual infected mice, and a CFU test for CRK was performed. Statistical significance with controls was analyzed using unpaired Student's t-test (***p < 0.001; **p < 0.01; *p < 0.05).
Fig. 4
Fig. 4. Effect of probiotics in a non-established CRK infection mouse model.
The effect of probiotics was evaluated in a mouse model in which CRK infection was not established. (B) Survival rates and (C) disease severity scores following probiotic treatment were evaluated for infection of two clinically isolated CRK strains. (D) All mice treated with probiotics were alive and healthy, whereas mice not treated with probiotics developed blood clots (left) and diarrhea (right) and even died. Statistical significance with controls was analyzed using unpaired Student's t-test (**p < 0.01); *p < 0.05).
Fig. 5
Fig. 5. Decolonization effect of probiotics on intestinal CRK.
(A) The decolonization effect of CRK colonized in the intestines of probiotics was evaluated in a CRK infection model that did not lead to death. CRK was infected after administration of a mixture of antibiotics (Met, methicillin; Kan, kanamycin; Van, vancomycin) to induce intestinal colonization of CRK. (B) Stool samples were continuously collected on days 2, 5, 8, and 14 during the 14-day observation period after infection, and CFU tests for CRK were performed. Statistical significance with controls was analyzed using unpaired Student's t-test (**p < 0.01, *p < 0.05).
Fig. 6
Fig. 6. Changes in stool pH following oral administration of L. fermentum in mice.
The pH value of the stool was measured while L. fermentum was administered orally for 2 weeks, and it was statistically compared with the control group through the unpaired Student's t-test (**p < 0.01, *p < 0.05).
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