Background: The health effects of e-cigarettes in patients with pre-existing lung disease are unknown. The aim of this study was to investigate whether aerosols from a fourth-generation e-cigarette produces similar in-vitro cytotoxic, DNA damage and inflammatory effects on bronchial epithelial cells (BECs) from patients with COPD, as cigarette smoke. VSports手机版.

Methods: BECs from patients with COPD who underwent surgery for lung cancer and comparator (immortalised 16HBE) cells were grown at air liquid interface (ALI). BECs were exposed to aerosols from a JUUL® e-cigarette (Virginia Tobacco and Menthol pods at 5% nicotine strength) or reference 3R4F cigarette for 30 min at ALI V体育安卓版. Cell cytotoxicity, DNA damage and inflammation were measured. .

Results: In response to the Virginia Tobacco and Menthol flavoured e-cigarette aerosols, COPD BECs showed comparable LDH release (cell cytotoxicity, p = 0. 59, p = 0. 67 respectively), DNA damage (p = 0. 41, p = 0. 51) and inflammation (IL-8, p = 0. 20, p = 0 V体育ios版. 89 and IL-6, p = 0. 24, p = 0. 93), to cigarette smoke. 16HBE cells also showed comparable cellular responses to cigarette smoke. .

Conclusion: In airway cells from patients with COPD, aerosols from a fourth-generation e-cigarette were associated with similar toxicity to cigarette smoke. These results have potential implications for the safety of e-cigarette use in patients with lung disease VSports最新版本. .