Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil VSports app下载. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 2021 Jun 15;21(1):119.
doi: 10.1186/s12902-021-00775-w.

The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS)

Zhi Wang  1 , Hui Dong  2 , Li Yang  1 , Ping Yi  2 , Qing Wang  3 , Dongmei Huang  4
Affiliations

The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS) (VSports在线直播)

V体育官网入口 - Zhi Wang et al. BMC Endocr Disord. .

Abstract

Background: To explore the development mechanism of PCOS and Transcriptomics was applied to seek the key gene VSports手机版. .

Methods: Transcriptomics marked by UID (unique identifier) technique of granulosa cell in PCOS and control women was carried out and key gene was picked up. Then the key gene in granulosa cell was measured by RT-PCR. Two PCOS models modeling with Letrozole and Testosterone Propionate were implemented and the key gene in granulosa cell of ovary was measured by immunohistochemistry to verify the relation with PCOS V体育安卓版. .

Results: GO-enrich of transcriptomics concentrated in domain steroid metabolism and domain mitochondria. Different genes were sought from coexisting in both domain steroid metabolism and domain mitochondria. Finally, five different genes including CYP11A1、CYB5R1、STAR、FDX1 and AMACR were obtained V体育ios版. RT-PCR was implemented to furtherly verify the downregulating mRNA of FDX1 in PCOS, which showed the consistent outcome with the transcriptomics. Level of FDX1 protein in granulosa cell of antral follicle in two PCOS models was measured and decreased. .

Conclusions: FDX1 was related with steroid metabolism and mitochondrial and may participate in the development of PCOS VSports最新版本. .

Keywords: FDX1; PCOS. V体育平台登录.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

VSports在线直播 - Figures

Fig. 1
Fig. 1
Differentially expressed gene. Volcano plot (A)、heatmap plot (B) and Bar plot (C)
Fig. 2
Fig. 2
Enrichment analysis including Go enrichment and KEGG enrichment according to data of UID-mRNA-sEq. Go enrichment (A: down-gene, B:up-gene) and KEGG enrichment (C: down-gene, D:up-gene)
Fig. 3
Fig. 3
Relative mRNA expression of FDX1 between PCOS women (n = 22) and control women (n = 21)
Fig. 4
Fig. 4
Expression of FDX1 in granulosa cell of antral follicle between normal group and model group by immunohistochemistry. Quantitative analysis was complemented (A) and length of GC layer was measured(B). Scale bar:50 μm. GC: granulosa cell. N: normal group; M: model group.(n = 3 for each group)
See this image and copyright information in PMC

References (V体育ios版)

    1. Anagnostis P, Tarlatzis BC, Kauffman RP. Polycystic ovarian syndrome (PCOS): Long-term metabolic consequences. Metabolism. 2018;86:33–43. doi: 10.1016/j.metabol.2017.09.016. - DOI - PubMed
    1. Dumesic DA, et al. Scientific Statement on the Diagnostic Criteria, Epidemiology, Pathophysiology, and Molecular Genetics of Polycystic Ovary Syndrome. Endocr Rev. 2015;36(5):487–525. doi: 10.1210/er.2015-1018. - DOI - PMC - PubMed
    1. Bremer AA, Miller WL. The serine phosphorylation hypothesis of polycystic ovary syndrome: a unifying mechanism for hyperandrogenemia and insulin resistance. Fertil Steril. 2008;89(5):1039–48. doi: 10.1016/j.fertnstert.2008.02.091. - V体育2025版 - DOI - PubMed
    1. Kahsar-Miller MD, et al. Prevalence of polycystic ovary syndrome (PCOS) in first-degree relatives of patients with PCOS. Fertil Steril. 2001;75(1):53–8. doi: 10.1016/S0015-0282(00)01662-9. - DOI - PubMed
    1. Matsuda F, et al. Follicular growth and atresia in mammalian ovaries: regulation by survival and death of granulosa cells. J Reprod Dev. 2012;58(1):44–50. doi: 10.1262/jrd.2011-012. - DOI - PubMed

MeSH terms