Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in VSports app下载. gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 2020 Oct 22:2020:3428587.
doi: 10.1155/2020/3428587. eCollection 2020.

"VSports在线直播" OPN Deficiency Increases the Severity of Osteoarthritis Associated with Aberrant Chondrocyte Senescence and Apoptosis and Upregulates the Expression of Osteoarthritis-Associated Genes

Jian Tian  1 , Chao Cheng  2 , Shi-Da Kuang  1 , Chao Su  1 , Xin Zhao  1 , Yi-Lin Xiong  1 , Yu-Sheng Li  1 , Shu-Guang Gao  1   3
Affiliations

"V体育安卓版" OPN Deficiency Increases the Severity of Osteoarthritis Associated with Aberrant Chondrocyte Senescence and Apoptosis and Upregulates the Expression of Osteoarthritis-Associated Genes

Jian Tian et al. Pain Res Manag. .

VSports最新版本 - Abstract

Objectives: A recent work has reported that the elevated osteopontin (OPN) levels in the articular cartilage and synovial fluid are correlated with the progressive osteoarthritis (OA) joint damage, and OPN has a protective effect against OA by suppressing the expressions of OA-associated genes VSports手机版. The present study examined whether the OPN deficiency was susceptible to OA through the regulation of chondrocyte senescence and apoptosis and the expressions of OA-associated genes. .

Methods: The mRNA levels of COL2A1 and OPN were compared between human OA chondrocytes and normal chondrocytes. The effects of OPN siRNA on the SA-β-Gal expressions and the percentage of apoptotic chondrocytes were examined by using SA-β-Gal staining and apoptosis assay, and the effects on the expressions of COL2A1 and OA-associated genes (COL10A1, IL-1β, TNF-ɑ, MMP-13, and ADAMTS5) were examined by western blot analysis and quantitative real-time RT-PCR V体育安卓版. Furthermore, an in vivo OA model was established to examine the effects of OPN siRNA on the senescence and apoptosis of OA chondrocytes and the expressions of OA-associated genes. .

Results: The mRNA levels of COL2A1 and OPN were decreased in knee OA chondrocytes in comparison with those in normal chondrocytes. The OPN deficiency enhanced the senescence and apoptosis of OA chondrocytes and increased the expressions of COL10A1, IL-1β, TNF-ɑ, MMP-13, and ADAMTS5 but decreased the expression of COL2A1. Meanwhile, OPN deficiency could result in severe, accelerated OA in vivo, which was also associated with enhanced senescence and apoptosis of chondrocytes and elevated expressions of OA-associated genes. V体育ios版.

Conclusions: The findings of this study suggest that the OPN deficiency can result in accelerated OA, which is associated with enhanced senescence and apoptosis of OA chondrocytes and the upregulated expressions of OA-associated genes. VSports最新版本.

PubMed Disclaimer

"VSports" Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Expressions of OPN and COL2A1 of the human OA chondrocytes and effects of OPN siRNA on the expression of osteoarthritis-associated genes. (a) The mRNA levels of OPN and COL2A1 in OA chondrocytes and in normal chondrocytes (∗p < 0.05 vs normal). (b) Western blot analysis was developed to evaluate the knockdown efficiency of OPN siRNA transfection. (c) Effects of OPN siRNA on the gene levels and protein levels of COL2A1, COL10A1, IL-1β, TNF-ɑ, MMP-13, and ADAMTS5 (∗p < 0.05 vs control or non-siRNA).
Figure 2
Figure 2
Effects of OPN siRNA on the senescence (a) and apoptosis (b) of OA chondrocytes (∗p < 0.05 vs control or non-siRNA).
Figure 3
Figure 3
In vivo study of the role of OPN deficiency in OA. (a) Immunohistochemistry was employed to evaluate the knockdown efficiency of OPN siRNA. (b) Safranin O staining of cartilage was performed and OARSI scores were compared among the three groups. (c) The expressions of SA-β-Gal, MMP-13, ADAMTS5, collagen type II, and aggrecan and the chondrocyte apoptosis percentage were compared among the three groups (∗p < 0.05 vs sham, #p < 0.05 vs DMM + nontargeting siRNA).
See this image and copyright information in PMC

References

    1. Cross M., Smith E., Hoy D., et al. The global burden of hip and knee osteoarthritis: estimates from the global burden of disease 2010 study. Annals of The Rheumatic Diseases. 2014;73(7):1323–1330. doi: 10.1136/annrheumdis-2013-204763. - DOI - PubMed
    1. Rahmati M., Nalesso G., Mobasheri A., et al. Aging and osteoarthritis: central role of the extracellular matrix. Ageing Research Reviews. 2017;40:20–30. doi: 10.1016/j.arr.2017.07.004. - DOI (V体育官网入口) - PubMed
    1. Yang H., Wen Y., Zhang M., et al. MTORC1 coordinates the autophagy and apoptosis signaling in articular chondrocytes in osteoarthritic temporomandibular joint. Autophagy. 2020;16(2):271–288. doi: 10.1080/15548627.2019.1606647. - DOI - PMC - PubMed
    1. Gao S. G., Zeng C., Li L. J., et al. Correlation between senescence-associated beta-galactosidase expression in articular cartilage and disease severity of patients with knee osteoarthritis. International Journal of Rheumatic Diseases. 2016;19(3):226–232. doi: 10.1155/2016/9561058. - DOI - PubMed
    1. Matsui Y., Iwasaki N., Kon S., et al. Accelerated development of aging-associated and instability-induced osteoarthritis in osteopontin-deficient mice. Arthritis & Rheumatology. 2009;60(8):2362–2371. doi: 10.1002/art.24705. - DOI - PubMed

Publication types

"VSports在线直播" MeSH terms