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. 2020 Oct 20:13:10641-10649.
doi: 10.2147/OTT.S261973. eCollection 2020.

Long Noncoding RNA CDKN2B-AS1 Facilitates Lung Cancer Development Through Regulating miR-378b/NR2C2

Guolei Wang  1 , Guanghui Xu  1 , Wenguang Wang  1
Affiliations

V体育2025版 - Long Noncoding RNA CDKN2B-AS1 Facilitates Lung Cancer Development Through Regulating miR-378b/NR2C2

Guolei Wang et al. Onco Targets Ther. .

Abstract

Aim: Long noncoding RNA (lncRNA) have proved to be important regulators in various diseases. CDKN2B-AS1 was a newly identified tumor-related lncRNA, and previous studies have reported its function in laryngeal squamous cancer and osteosarcoma VSports手机版. However, the function and mechanism of lncRNA CDKN2B-AS1 in lung cancer are still unknown. .

Methods: Cell proliferation, invasion, migration and apoptosis were detected via CCK-8, transwell assay and Western blot V体育安卓版. Bioinformatics analysis was used to predict the potential target of CDKN2B-AS1. A rescue experiment was performed to identify the relationship between CDKN2B-AS1 and miR-378b. .

Results: The expression of lncRNA CDKN2B-AS1 was significantly upregulated in lung cancer tissues and cell lines. Overexpression of CDKN2B-AS1 promoted cell proliferation, invasion and reduced cell apoptosis. Knockdown of CDKN2B-AS1 inhibited cell proliferation, invasion and increased cell apoptosis. Bioinformatics analysis predicted that miR-378b was the direct target. We also provided evidence that NR2C2 was the target of miR-378b. The expression of NR2C2 was significantly upregulated in lung cancer tissues and cell lines. The rescue experiment further confirmed the relationship between CDKN2B-AS1 and miR-378b V体育ios版. Overexpression of miR-378b completely reversed the function of CDKN2B-AS1. .

Conclusion: Taken together, our results comprehensively analyzed the function of CDKN2B-AS1 in lung cancer and provided a possible mechanism that CDKN2B-AS1 facilitates lung cancer development by regulating miR-378b and NR2C2. Thus, our study offers a potential therapeutic target for treating lung cancer VSports最新版本. .

Keywords: CDKN2B-AS1; lncRNA; lung cancer. V体育平台登录.

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Conflict of interest statement (VSports最新版本)

The authors declare no competing financial or non-financial interests for this work.

Figures

Figure 1
Figure 1
Biological features of lncRNA CDKN2B-AS1. (A) The expression of lncRNA CDKN2B-AS1 was increased in lung cancer tissues (N=20). (B) The expression of miR-378b was decreased in lung cancer tissues (N=20). (C) The expression of lncRNA CDKN2B-AS1 was increased in lung cancer cell lines. (D) The expression of miR-378b was decreased in lung cancer cell lines. **P<0.01.
Figure 2
Figure 2
Overexpression of lncRNA CDKN2B-AS1 promoted lung cancer development. (A) Overexpression was verified by real-time PCR. (B) Cell invasion was assessed by transwell. (C) Overexpression of lncRNA CDKN2B-AS1 promoted cell proliferation in A549 and H1299 cells. (D) Cell proliferation and apoptosis markers were measured by Western blot. *P<0.05, **P<0.01, ***P<0.001.
Figure 3
Figure 3
Knockdown of lncRNA CDKN2B-AS1 inhibited lung cancer development. (A) Knockdown was verified by real-time PCR. (B) Cell invasion was assessed by transwell. (C) Knockdown of lncRNA CDKN2B-AS1 inhibited cell proliferation in A549 and H1299 cells. (D) Cell proliferation and apoptosis markers were measured by Western blot. **P<0.01, ***P<0.001.
Figure 4
Figure 4
miR-378b was the direct target of CDKN2B-AS1. (A) CDKN2B-AS1 and miR-378b located in cytoplasm. (B) The potential binding sequence between CDKN2B-AS1 and miR-378b was shown. (C) Overexpression of miR-378b was verified by real-time PCR. (D) Dual-luciferase reporter was performed to identify the relationship between miR-378b and CDKN2B-AS1. (E) Overexpression of CDKN2B-AS1 reduced the expression of miR-378b and vice versa. (F) CDKN2B-AS1 negatively regulated the expression of miR-378b. (G) Overexpression of miR-378b inhibited cell proliferation in A549 and H1299 cells. (H) Overexpression of miR-378b reduced the cell invasion. **P<0.01, ***P<0.001.
Figure 5
Figure 5
NR2C2 was the direct target of miR-378b. (A) The potential binding sequence between NR2C2 and miR-378b was shown. (B) Dual-luciferase reporter was performed to identify the relationship between miR-378b and NR2C2. (C) Overexpression of miR-378b reduced the expression of NR2C2 by real-time PCR. (D) Overexpression of miR-378b reduced the expression of NR2C2 by Western blot. (E) The expression of NR2C2 was increased in lung cancer tissues. (N=20) (F) Overexpression of miR-378b blocked the effect of CDKN2B-AS1 by Western blot. (G) Overexpression of miR-378b blocked the effect of CDKN2B-AS1 by real-time PCR. (H) CDKN2B-AS1 positively regulated the expression of NR2C2. *P<0.05, **P<0.01.
Figure 6
Figure 6
lncRNA CDKN2B-AS1 facilitates cancer progress through regulating miR-378b. (A) Overexpression of miR-378b blocked the cell invasion induced by lncRNA CDKN2B-AS1. (B) Quantification data were shown. (C) Overexpression of miR-378b rescued the pro-proliferation effect of CDKN2B-AS1 in H1299 and A549 cells. (D) Proliferation and apoptosis markers were measured via Western blot. (E) Quantification data were shown. *P<0.05, **P<0.01, ***P<0.001, #CDKN2B-AS1 VS CDKN2B-AS1+miR-378b, P<0.05.
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