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. 2020 Sep 7;11(1):384.
doi: 10.1186/s13287-020-01902-5.

Adipose-derived mesenchymal stem cells attenuate acute lung injury and improve the gut microbiota in septic rats

Junyi Sun  1   2 , Xianfei Ding  1   2 , Shaohua Liu  1 , Xiaoguang Duan  1 , Huoyan Liang  1   2 , Tongwen Sun  3
Affiliations

V体育平台登录 - Adipose-derived mesenchymal stem cells attenuate acute lung injury and improve the gut microbiota in septic rats

Junyi Sun et al. Stem Cell Res Ther. .

Abstract

Background: We hypothesized that adipose-derived mesenchymal stem cells (ADMSCs) may ameliorate sepsis-induced acute lung injury (ALI) and change microorganism populations in the gut microbiota, such as that of Firmicutes and Bacteroidetes VSports手机版. .

Methods: A total of 60 male adult Sprague-Dawley (SD) rats were separated into three groups: the sham control (SC) group, the sepsis induced by cecal ligation and puncture (CLP) group, and the ADMSC treatment (CLP-ADMSCs) group, in which rats underwent the CLP procedure and then received 1 × 106 ADMSCs. Rats were sacrificed 24 h after the SC or CLP procedures V体育安卓版. To study the role of ADMSCs during ALI caused by sepsis and examine the impact of ADMSCs on the gut microbiome composition, rat lungs were histologically evaluated using hematoxylin and eosin (H&E) staining, serum levels of pro-inflammatory factors were detected using enzyme-linked immunosorbent assay (ELISA), and fecal samples were collected and analyzed using 16S rDNA sequencing. .

Results: The serum levels of inflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-6, were significantly increased in rats after the CLP procedure, but were significantly decreased in rats treated with ADMSCs. Histological evaluation of the rat lungs yielded results consistent with the changes in IL-6 levels among all groups. Treatment with ADMSCs significantly increased the diversity of the gut microbiota in rats with sepsis. The principal coordinates analysis (PCoA) results showed that there was a significant difference between the gut microbiota of the CLP-ADMSCs group and that of the CLP group. In rats with sepsis, the proportion of Escherichia-Shigella (P = 0. 01) related to lipopolysaccharide production increased, and the proportion of Akkermansia (P = 0 V体育ios版. 02) related to the regulation of intestinal mucosal thickness and the maintenance of intestinal barrier function decreased. These changes in the gut microbiota break the energy balance, aggravate inflammatory reactions, reduce intestinal barrier functions, and promote the translocation of intestinal bacteria. Intervention with ADMSCs increased the proportion of beneficial bacteria, reduced the proportion of harmful bacteria, and normalized the gut microbiota. .

Conclusions: Therapeutically administered ADMSCs ameliorate CLP-induced ALI and improves gut microbiota, which provides a potential therapeutic mechanism for ADMSCs in the treatment of sepsis VSports最新版本. .

Keywords: Acute lung injury; Cecal ligation and puncture; Gut microbiota; Mesenchymal stem cells; Sepsis. V体育平台登录.

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Conflict of interest statement

The authors have no conflicts of interest to disclose.

Figures

Fig. 1
Fig. 1
Effect of adipose-derived mesenchymal stem cells (ADMSCs) on systemic inflammatory response in rats with cecal ligation and puncture (CLP)-induced acute lung injury (ALI). The serum levels of IL-6 (a) and TNF-α (b) were measured using enzyme-linked immunosorbent assay (ELISA). *Compared with the sham control (SC) group; #compared with the CLP group; *, #P < 0.05; **, ##P < 0.01; ***, ###P < 0.001. c Effect of CLP and ADMSCs on 24-h lung histology. Hematoxylin and eosin staining showed that inflammatory infiltration, edema, and hemorrhage of rat lung tissue from the CLP group were significantly enhanced. Treatment with ADMSCs improved these anomalies (bar = 100 μm)
Fig. 2
Fig. 2
Abundance analysis of intestinal microecology. The abscissa represents the number of randomly selected sequences. The ordinate represents the number of operational taxonomic units (OTUs) observed
Fig. 3
Fig. 3
a Principal coordinates analysis (PCoA) based on unweighted UniFrac values of rats from the different treatment groups. b Principal component analysis (PCA) of rats from the different treatment groups
Fig. 4
Fig. 4
a Distribution of phylum in the gut microbiota of rats from the different treatment groups. b Distribution of genus in the gut microbiota of rats from the different treatment groups. d Cluster graphs. Left: The Bray-Curtis distance clustering tree structure. Closer sample clustering and shorter branches indicate a higher degree of similarity in the sample species composition. Right: The relative abundance distribution map of each sample at the gate level. A larger proportion indicates higher abundance. d Heat map. The gradient colors from blue to red reflect the change in abundance from low to high. Colors closer to blue indicate a lower abundance; colors closer to red indicate a higher abundance
See this image and copyright information in PMC

References

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