"V体育安卓版" Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or VSports app下载. mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2020 Apr 23:2020:4897983.
doi: 10.1155/2020/4897983. eCollection 2020.

V体育ios版 - Synovial Fluid Cell Proteomic Analysis Identifies Upregulation of Alpha-Taxilin Proteins in Rheumatoid Arthritis: A Potential Prognostic Marker

Ashish Sarkar  1 , Shivani Sharma  1 , Prachi Agnihotri  1 , Tanmoy Sarkar  1 , Pooja Kumari  1 , Rajesh Malhotra  2 , Barun Datta  3 , Vijay Kumar  2 , Sagarika Biswas  1
Affiliations

Synovial Fluid Cell Proteomic Analysis Identifies Upregulation of Alpha-Taxilin Proteins in Rheumatoid Arthritis: A Potential Prognostic Marker

Ashish Sarkar (VSports注册入口) et al. J Immunol Res. .

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease affecting the joints and surrounding tissue. Identification of novel proteins associated with the progression of a disease is a prerequisite for understanding the pathogenesis of RA. The present study was undertaken to identify the potential biomarkers from a less explored biological sample such as synovial fluid (SF) cells which is specific for RA and to analyze their functional aspects using proteomic approach. Two-dimensional gel electrophoresis (2-DE) was performed using synovial fluid cells of RA and osteoarthritis (OA) patients, and 7 differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS). Αlpha-Taxilin (α-Taxilin) has been found as one of the novel, significantly up regulated protein in RA. It has been validated in the synovium, synovial fluid (SF), SF cells, and plasma samples by Western blot, enzyme-linked immunosorbent assay (ELISA), fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and real-time PCR VSports手机版. The identification of autoantibody against α-Taxilin and in silico studies has further helped us to understand its involvement in disease mechanism. The present study will therefore provide knowledge towards the etiology of RA that pave the way for suitable prognostic marker identification along with other clinical parameters. .

PubMed Disclaimer

Conflict of interest statement

The authors confirm that this article content has no conflicts of interest.

VSports - Figures

Figure 1
Figure 1
Differential proteome profile. Protein profile of synovial fluid cells of RA and OA showing differentially expressed protein spots in synovial fluid cell lysate using 2D-SDS PAGE gel. 150 μg of protein sample was loaded in IPG (pH 4-7) strip for 2D SDS-PAGE followed by silver staining.
Figure 2
Figure 2
Validation by Western blot. Graph showing mean densitometric values obtained after Western blot analysis in different biological samples. (a) Expression level of α-Taxilin in synovial fluid cells of RA and OA. (b) Expression level of in the synovium of RA and OA. (c) Expression level of α-Taxilin in blood plasma of RA, OA, and HC. (d) Expression level of α-Taxilin in synovial fluid of RA and OA.
Figure 3
Figure 3
Validation by Elisa. ELISA was performed using plasma (RA = 100, OA = 100, and HC = 64) and SF (RASF = 16 and OASF = 16) sample using anti-α-Taxilin antibody followed by HRP-conjugated secondary antibody. Graph showing mean absorbance of ELISA at 492 nm. (a) Graph showing the α-Taxilin level in RA plasma sample compared to OA plasma sample and healthy controls (HC). (b) Graph showing the α-Taxilin level in SF sample of RA compared to OA.
Figure 4
Figure 4
Validation by FACS. Blood cells or SF cells from RA, OA, and healthy blood cells were isolated and proceeded for FACS analysis with α-Taxilin primary antibody followed by Alexa Fluor 467-tagged secondary antibody. (a) Data captured using FACS caliber showing percentage of cells positively stained with Alexa Fluor 467 in H, OA, and RA blood cells. (b) Graphical representation of FACS data in terms of cells stained with Alexa Fluor for H, OA, and RA samples. (c) Data captured using FACS caliber showing the percentage of cells positively stained with Alexa Fluor 467 in SF cells. (d) Graphical representation of FACS data in terms of cells stained with Alexa Fluor for OA and RA SF cells.
Figure 5
Figure 5
Expression of α-Taxilin in the synovium. (a) Tissue section were cut and stained with anti α-Taxilin primary antibody followed by HRP-tagged secondary antibody and subsequently developed by DAB then an image taken at 20x and 100x shows high expression of α-Taxilin protein in the synovium of RA as compared to OA and healthy control. Pink color represents the level of α-Taxilin in the synovium sample. (b) Comparative densitometric analysis of Western blot of the pool sample showing the level of circulating autoantibody against α-Taxilin in RA, OA, and healthy plasma.
Figure 6
Figure 6
“STRING” pathway analysis and interaction study. In silico study of interacting partner of α-Taxilin protein using string pathway analysis. The binding affinity of the proteins is shown in Homo sapiens as well as in other species in terms of the association score.
See this image and copyright information in PMC

"VSports最新版本" References

    1. Smolen J. S., Aletaha D., Koeller M., Weisman M. H., Emery P. New therapies for treatment of rheumatoid arthritis. The Lancet. 2007;370(9602):1861–1874. doi: 10.1016/S0140-6736(07)60784-3. - DOI - PubMed
    1. Gonzalez A., Maradit Kremers H., Crowson C. S., et al. The widening mortality gap between rheumatoid arthritis patients and the general population. Arthritis and Rheumatism. 2007;56(11):3583–3587. doi: 10.1002/art.22979. - DOI - PubMed
    1. Firestein G. S. Evolving concepts of rheumatoid arthritis. Nature. 2003;423(6937):356–361. doi: 10.1038/nature01661. - "V体育平台登录" DOI - PubMed
    1. Biswas S., Sharma S., Saroha A., et al. Identification of novel autoantigen in the synovial fluid of rheumatoid arthritis patients using an immunoproteomics approach. PLoS One. 2013;8(2, article e56246) doi: 10.1371/journal.pone.0056246. - VSports最新版本 - DOI - PMC - PubMed
    1. Korganow A. S., Ji H., Mangialaio S., et al. From systemic T cell self-reactivity to organ-specific autoimmune disease via immunoglobulins. Immunity. 1999;10(4):451–461. doi: 10.1016/S1074-7613(00)80045-X. - DOI - PubMed

MeSH terms

"V体育官网" LinkOut - more resources