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. 2019 Jan;20(1):61-68.
doi: 10.2174/1389202919666181002144336.

V体育平台登录 - Profile of HBV Integration in the Plasma DNA of Hepatocellular Carcinoma Patients

Weiyang Li  1 , Xiaofang Cui  1 , Qing Huo  1 , Yanwei Qi  1 , Yuhui Sun  1 , Meihua Tan  1 , Qingsheng Kong  1
Affiliations

"V体育安卓版" Profile of HBV Integration in the Plasma DNA of Hepatocellular Carcinoma Patients

Weiyang Li et al. Curr Genomics. 2019 Jan.

Abstract (V体育官网)

Background: Hepatitis B Viral (HBV) infection is one of the major causes of Hepatocellular Carcinoma (HCC). Mounting evidence had provided that the HBV integration might be a critical con-tributor of HCC carcinogenesis. VSports手机版.

Objective and methods: To explore the profile of HBV integration in the plasma DNA, the method of next-generation sequencing, HBV capture and bioinformatics had been employed to screen for HBV in-tegration sites in the plasma samples. V体育安卓版.

Results: In the initial experiment, a total of 87 breakpoints were detected in the 20 plasma samples V体育ios版. The distribution of breakpoints showed that there was significant enrichment of breakpoints in the region of intron. Furthermore, the HBV breakpoints were prone to occur in the region of X protein (1,700-2,000bp) in the plasma samples. The pathway analysis had revealed that the HBV integrations sites were specifically enriched in the cancer pathway. .

Conclusion: Altogether, our results had provided direct evidence for the HBV integration in plasma DNA, and they might be potentially useful for future HCC prognosis and diagnosis VSports最新版本. .

Keywords: Breakpoints; Cell free DNA; HBV genome; HBV integration; Hepatocellular carcinoma; Plasma V体育平台登录. .

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V体育官网 - Figures

Fig. (1)
Fig. (1)
The distribution of breakpoints in gene elements and HBV genome. (a) Observed represented the observed ratio of breakpoints; Random represented the random ratio of breakpoints. NS represented no significant. (b) Histograms were constructed for 100-bp intervals. HBV genes with different functions are marked. The number of breakpoints located in the HBV genome was shown.
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