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. 2018 Sep 1;8(9):1775-1787.
eCollection 2018.

V体育2025版 - Lung tissue microbial profile in lung cancer is distinct from emphysema

Yanhong Liu  1   2 , Jacqueline L O'Brien  3   4 , Nadim J Ajami  3   4 , Michael E Scheurer  1   5 , E Susan Amirian  1 , Georgina Armstrong  1 , Spiridon Tsavachidis  1 , Aaron P Thrift  1   2 , Li Jiao  1   6 , Matthew C Wong  3   4 , Daniel P Smith  3   4 , Margaret R Spitz  1   2 , Melissa L Bondy  1   2 , Joseph F Petrosino  3   4 , Farrah Kheradmand  1   2   6
Affiliations

Lung tissue microbial profile in lung cancer is distinct from emphysema

Yanhong Liu et al. Am J Cancer Res. .

Abstract

Objectives: The composition and structure of site-specific microbiota have been investigated as potential biomarkers for a variety of chronic inflammatory diseases and cancers. While many studies have focused on the changes in the airway microbiota using respiratory specimens from patients with various respiratory diseases, more research is needed to explore the microbial profiles within the distal lung parenchyma in smokers with lung cancer and/or emphysema VSports手机版. .

Materials and methods: To describe and contrast lung tissue-associated microbial signatures in smokers with lung cancer and/or emphysema, we employed culture-independent pyrosequencing of 16S rRNA gene hypervariable V4 region and compositional analysis in non-malignant lung tissue samples obtained from 40 heavy smokers, including 10 emphysema-only, 11 lung cancer-only, and 19 with both lung cancer and emphysema V体育安卓版. .

Results and conclusion: The emphysema-only group presented a lower bacterial community evenness defined by a significantly lower Shannon diversity index compared to the lung cancer patients with or without emphysema (P = 0. 006). Furthermore, community compositions of lung cancer patients with or without emphysema were characterized by a significantly lower abundance of Proteobacteria (primary the genera Acinetobacter and Acidovorax) and higher prevalence of Firmicutes (Streptococcus) and Bacteroidetes (Prevotella), compared to emphysema-only patients. In conclusion, the lung microbial composition and communities structures of smokers with lung cancer are distinct from the emphysema-only patients. Although preliminary, our findings suggest that lung microbiome changes could be a biomarker of lung cancer that could eventually be used to help screening for the disease. V体育ios版.

Keywords: 16S rRNA gene; Lung cancer; bacteria; emphysema; microbiome. VSports最新版本.

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"V体育安卓版" Conflict of interest statement

None.

Figures

Figure 1
Figure 1
Alpha-diversity of the lung bacterial communities in LC and emphysema patients. A. Number of observed OTUs (operational taxonomic units), a measure of species richness; B. Shannon diversity index, a measure of species richness and evenness. The P values of the Kruskal-Wallis test refer to the comparison among the three groups: emphysema-only (n = 10, red circles), LC only (n = 11, blue circles), and both LC and emphysema (n = 19, green circles). The P values of the Wilcoxon rank-sum test, refer to the comparison between emphysema-only (red circles, n = 10) and LC patients with or without emphysema (blue and green circles, n = 30).
Figure 2
Figure 2
Smoking PPY and observed OTUs. A. In all samples (N = 40): Although not significant, the number of observed OTUs (operational taxonomic units) decreased as the number of PPY (pack-year) increased, with the Kruskal-Wallis test P-trend of 0.36. B. Among the three groups: the Kruskal-Wallis test P-trend were 0.54, 0.14 and 0.71, for emphysema-only (n = 10), LC-only (n = 11), and both LC and emphysema patients (n = 19), respectively.
Figure 3
Figure 3
Principal coordinate analysis of lung bacterial communities based on weighted Unifrac distances. Each symbol represents a sample. The proportion of variance explained by each principal component is denoted in the corresponding axis label. These two components explained 52.6.1% of the variance. The lung bacterial communities of the LC patients, with (green circles) or without (blue circles) emphysema, were clustered together, and distinctly separated from the emphysema-only patients (red circles). Both P values from the Kruskal-Wallis test (comparison among three groups) and the Wilcoxon rank-sum test (emphysema-only versus LC patients), were 0.001.
Figure 4
Figure 4
Relative abundance of bacterial genera in the lung communities among the three groups. A. Phylum level; B. Genus level. The colors represent the mean relative abundance (0 to 1, blue to red) of each genus among three groups: emphysema-only (n = 10, red squares), LC-only (n = 11, blue squares), and both LC and emphysema (n = 19, green squares). The asterisks indicates significantly different taxa.
Figure 5
Figure 5
Bacterial distribution patterns in lung tissue and other body sites. A. Phylum-level bacteria in lung tissue among three groups; B. Phylum- and genus-level bacteria in healthy human subjects body sites from HMP (cited from [45]). The proportions of the mean relative abundance of each genus are shown.
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