Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2018 Sep 25;2(10):1199-1212.
doi: 10.1002/hep4.1204. eCollection 2018 Oct.

Reactive Ductules Are Associated With Angiogenesis and Tumor Cell Proliferation in Pediatric Liver Cancer

Sanghoon Lee  1 , Ping Zhou  1 , Anita Gupta  2 , Soona Shin  1
Affiliations

Reactive Ductules Are Associated With Angiogenesis and Tumor Cell Proliferation in Pediatric Liver Cancer

"VSports app下载" Sanghoon Lee et al. Hepatol Commun. .

"VSports在线直播" Abstract

While reactive ductules (RDs) have been observed in viral hepatitis, biliary atresia, nonalcoholic fatty liver disease, and adult hepatocellular carcinoma (HCC), RDs in pediatric liver cancer remain uncharacterized. This study investigated the relationship of RDs with angiogenic paracrine factors, the extent of angiogenesis, and tumor cell proliferation in pediatric hepatoblastoma (HBL)/HCC livers. We quantified the extent of RDs and their expression of paracrine factors that include vascular endothelial growth factor (VEGF), vascular endothelial growth factor D (VEGFD), platelet-derived growth factor C, and angiopoietin 1 (ANGPT1). In addition, we performed immunohistochemical detection of the endothelial marker clusters of differentiation (CD)34 and the proliferation marker Ki67 followed by correlation analyses. In HBL, we found the percentage of RDs with Ki67 expression (% Ki67+ RDs) significantly correlated with intratumoral Ki67+ areas (r = 0. 5138, P = 0. 0349) and % ANGPT1+ RDs positively correlated with % Ki67+ RDs (r = 0. 5851, P = 0. 0136). In HCC, the high ANGPT1+ RDs group (i. e. , cases with % ANGPT1+ RDs ≥50) exhibited high intratumoral Ki67+ areas compared to the low ANGPT1+ RDs group. In the combined HBL and HCC liver tumor group, there was a positive association between % platelet-derived growth factor C+ RDs and intratumoral Ki67+ areas (r = 0 VSports手机版. 4712, P = 0. 0099) and the high VEGFD+ RDs group (≥50%) exhibited a high number of peritumoral CD34+ vessels compared to the low VEGFD+ RDs group. Conclusion: Paracrine factor-expressing RDs are associated with angiogenesis and proliferation of pediatric liver tumors. .

PubMed Disclaimer

Figures

Figure 1
Figure 1
Representative immunostaining of EpCAM+ RDs in HBL and HCC. RDs are detected in peritumoral areas in (A,B) HBL and (C,D) HCC livers. Tumor borders are marked with dashed lines.
Figure 2
Figure 2
RDs positive for VEGF, VEGFD, and ANGPT1 are detected in the peritumoral area (HBL). (A) RDs in the peritumoral area are identified by EpCAM staining. Shown are subsequent serial sections of the same peritumoral area for VEGF+ RDs, VEGFD+ RDs, ANGPT1+ RDs, and Ki67+ RDs. (B) CD34+ cells in peritumoral and intratumoral areas are identified in a subsequent serial section of the same area. Tumor borders are marked with dashed lines. Abbreviations: ITA, intratumoral area; PTA, peritumoral area.
Figure 3
Figure 3
RDs positive for VEGF, VEGFD, and PDGFC are detected in the peritumoral area (HCC). (A) RDs in the peritumoral area are identified by EpCAM+ staining. Shown are subsequent serial sections of the same peritumoral area for VEGF+ RDs, VEGFD+ RDs, PDGFC+ RDs, and CD34+ vessels. (B) Ki67+ cells in the peritumoral area are identified. Arrows indicate EpCAM+Ki67+ RDs in serial sections of the same area. Tumor borders are marked with dashed lines. Abbreviation: PTA, peritumoral area.
See this image and copyright information in PMC

References

    1. Emre S, Umman V, Rodriguez‐Davalos M. Current concepts in pediatric liver tumors. Pediatr Transplant 2012;16:549‐563. - PubMed (V体育官网)
    1. Darbari A, Sabin KM, Shapiro CN. Schwarz KB. Epidemiology of primary hepatic malignancies in U.S. children. Hepatology 2003;38:560‐566. - PubMed
    1. Litten JB, Tomlinson GE. Liver tumors in children. Oncologist 2008;13:812‐820. - PubMed
    1. Allan BJ, Parikh PP, Diaz S, Perez EA, Neville HL, Sola JE. Predictors of survival and incidence of hepatoblastoma in the paediatric population. HPB (Oxford) 2013;15:741‐746. - VSports app下载 - PMC - PubMed
    1. Allan BJ, Wang B, Davis JS, Parikh PP, Perez EA, Neville HL, et al. A review of 218 pediatric cases of hepatocellular carcinoma. J Pediatr Surg 2014;49:166‐171. - PubMed