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. 2018 Apr 23;11(1):31.
doi: 10.1186/s13048-018-0406-z.

Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing (VSports最新版本)

Qianying Zhao  1   2 , Qiuhong Qian  1   3 , Dongyan Cao  1 , Jiaxin Yang  1 , Ting Gui  4 , Keng Shen  5
Affiliations

"VSports注册入口" Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing

"V体育官网" Qianying Zhao et al. J Ovarian Res. .

Abstract

Background: B-cell-specific Moloney murine leukemia virus integration site 1 (BMI1) might be an appropriate biomarker in the management of epithelial ovarian cancer (EOC). However, the biological role of BMI1 and its relevant molecular mechanism needs further elaboration VSports手机版. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is an excellent genome-editing tool and is scarcely used in EOC studies. .

Methods: We first applied CRISPR/Cas9 technique to silence BMI1 in EOC cells; thereafter we accomplished various in vivo and in vitro experiments to detect biological behaviors of ovarian cancer cells, including MTT, flow cytometry, Transwell, real-time polymerase chain reaction and western blotting assays, etc V体育安卓版. ; eventually, we used RNA sequencing to reveal the underlying molecular traits driven by BMI1 in EOC. .

Results: We successfully shut off the expression of BMI1 in EOC cells using CRISPR/Cas9 system, providing an ideal cellular model for investigations of target gene. Silencing BMI1 could reduce cell growth and metastasis, promote cell apoptosis, and enhance the platinum sensitivity of EOC cells. BMI1 might alter extracellular matrix structure and angiogenesis of tumor cells through regulating Focal adhesion and PI3K/AKT pathways V体育ios版. .

Conclusion: BMI1 is a potential biomarker in EOC management, especially for tumor progression and chemo-resistance. Molecular traits, including BMI1 and core genes in Focal adhesion and PI3K/AKT pathways, might be alternatives as therapeutic targets for EOC. VSports最新版本.

Keywords: B-cell-specific Moloney murine leukemia virus integration site 1 (BMI1); Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9; Epithelial ovarian cancer (EOC); Focal adhesion pathway; PI3K/AKT pathway. V体育平台登录.

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Conflict of interest statement

Ethics approval and consent to participate

All mice were housed and fed under specific pathogen-free conditions in an institution approved by the Beijing Laboratory Animal Research Center VSports注册入口. All studies were approved and supervised by Peking Union Medical College Hospital.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

"V体育官网" Figures

Fig. 1
Fig. 1
a. BMI1 gRNAs successfully inserted into pX330-BbsI vectors; b. Sub-clones with shortened nucleic acid sequences on Agarose gel after PCR; c. Sub-clones with no expression of BMI1 protein entirely detected by western blotting
Fig. 2
Fig. 2
Changes of Biological behaviors after silencing BMI1: a. cell growth curve; b. cell cycle pattern; c. Transwell invasion; d. Transwell migration; e. in vivo experiments; f. apoptosis markers; g. chemotherapeutic responses
Fig. 3
Fig. 3
Validation of RNA sequencing results by a. RT-PCR; b. western blotting
See this image and copyright information in PMC

VSports手机版 - References

    1. Tangjitgamol S, Manusirivithaya S, Laopaiboon M, Lumbiganon P. Interval debulking surgery for advanced epithelial ovarian cancer: a Cochrane systematic review. Gynecol Oncol. 2009;112(1):257–264. doi: 10.1016/j.ygyno.2008.09.041. - DOI - PubMed
    1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65(2):87–108. doi: 10.3322/caac.21262. - DOI - PubMed
    1. Tanda ET, Budroni M, Cesaraccio R, Palmieri G, Palomba G, Capobianco G, et al. Epidemiology of ovarian cancer in North Sardinia, Italy, during the period 1992-2010. Eur J Gynaecol Oncol. 2015;36(1):69–72. - "VSports注册入口" PubMed
    1. Bracken AP, Kleine-Kohlbrecher D, Dietrich N, Pasini D, Gargiulo G, Beekman C, et al. The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells. Genes Dev. 2007;21(5):525–530. doi: 10.1101/gad.415507. - DOI - PMC - PubMed
    1. Siddique HR, Saleem M. Role of BMI1, a stem cell factor, in cancer recurrence and chemoresistance: preclinical and clinical evidences. Stem Cells. 2012;30(3):372–378. doi: 10.1002/stem.1035. - DOI - PubMed

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