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. 2018 Apr 1:418:97-108.
doi: 10.1016/j.canlet.2018.01.022. Epub 2018 Jan 10.

Down-regulation of RIP3 potentiates cisplatin chemoresistance by triggering HSP90-ERK pathway mediated DNA repair in esophageal squamous cell carcinoma

Yulin Sun  1 , Linhui Zhai  2 , Shouzhi Ma  1 , Chengpu Zhang  3 , Lina Zhao  1 , Ning Li  3 , Yang Xu  1 , Tao Zhang  3 , Zhimin Guo  1 , Heng Zhang  3 , Ping Xu  4 , Xiaohang Zhao  5
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"VSports手机版" Down-regulation of RIP3 potentiates cisplatin chemoresistance by triggering HSP90-ERK pathway mediated DNA repair in esophageal squamous cell carcinoma

Yulin Sun et al. Cancer Lett. .

Abstract

Receptor interacting protein kinase 3 (RIP3) is a critical regulator of programmed necrotic cell death. Here, we observed that RIP3 was significantly down-regulated in esophageal cancer. And its remaining expression was associated with better response to chemotherapy and prolonged survival. Notably, re-expression of kinase-dead RIP3 also restored cisplatin sensitivity, suggesting that some roles of RIP3 beyond necroptosis may be involved in cisplatin-based chemosensitivity. To investigate the mechanisms, a large-scale quantitative proteomics study was performed after cisplatin treatment in RIP3-knockdown cells VSports手机版. In total, approximately 7000 protein groups were confidently identified, with a false discovery rate of 0. 21% at the protein level. Of these proteins, 685 displayed RIP3-dependent changes in abundance. Bioinformatics analyses indicated that DNA repair pathway was stimulated after RIP3 depletion. Functional studies showed that deficient RIP3 upregulated FOSL1 and POLD1 through activation of the HSP90/CDC37 complex and ERK phosphorylation in multiple cell lines. Furthermore, via inhibition of the HSP90/CDC37 complex, ERK and FOSL1 reversed the cisplatin resistance phenotype. These results suggest that RIP3 regulates cisplatin sensitivity through both pronecrotic and non-necrotic functions. RIP3 may be a potential marker for predicting chemosensitivity. .

Keywords: Chemosensitivity; DNA repair; HSP90/CDC37 complex; RIP3; iTRAQ. V体育安卓版.

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