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Observational Study
. 2017 Nov 3:12:3237-3244.
doi: 10.2147/COPD.S144969. eCollection 2017.

SIRT1 and FOXO1 mRNA expression in PBMC correlates to physical activity in COPD patients (V体育官网入口)

Chihiro Taka  1 , Ryuji Hayashi  1 , Kazuki Shimokawa  1 , Kotaro Tokui  1 , Seisuke Okazawa  1 , Kenta Kambara  1 , Minehiko Inomata  1 , Toru Yamada  1 , Shoko Matsui  1 , Kazuyuki Tobe  1
Affiliations
Observational Study

SIRT1 and FOXO1 mRNA expression in PBMC correlates to physical activity in COPD patients

Chihiro Taka et al. Int J Chron Obstruct Pulmon Dis. .

V体育2025版 - Abstract

Background: Physical activity (PA) is considered as one of the most important prognostic predictors in chronic obstructive pulmonary disease (COPD) patients. Longevity gene, SIRT1, is reported to be involved in the pathogenesis of COPD by regulating the signaling pathways of oxidative stress, inflammation, and aging. We hypothesize that SIRT1 and related genes are also associated with the benefits of PA in COPD patients VSports手机版. .

Methods: Eighteen COPD outpatients were enrolled in this study, and their PA level was assessed with an accelerometer V体育安卓版. We assessed the SIRT1 and related genes mRNA expression levels in the peripheral blood mononuclear cells (PBMCs) of the subjects. We carried out respiratory function testing, blood gas analysis, the 6-minute walk test, and measurement of the cross-sectional area of the erector spinae muscles (ESMCSA) by chest computed tomography. We analyzed the association of PA with the results of each of the examinations. .

Results: The mean age was 72±9 years, and the mean forced expiratory volume in 1 second was 1. 4±0. 56 L (52%±19% predicted). Our findings revealed a correlation between the daily PA and ESMCSA. The SIRT1 and Forkhead box O (FOXO)1 mRNA expression levels in PBMCs were positively correlated with moderate-PA time (r=0 V体育ios版. 60, p=0. 008 for SIRT1 and r=0. 59, p=0. 01 for FOXO1). .

Keywords: COPD; accelerometer; mRNA; moderate; sedentary; walking VSports最新版本. .

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Conflict of interest statement (V体育2025版)

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Time spent in various PA intensities. Notes: Sedentary is 1–1.9 METs; light is 2–2.9 METs; moderate is 3–5.9 METs; and vigorous is ≥6 METs. Abbreviations: PA, physical activity; METs, metabolic equivalents.
Figure 2
Figure 2
The relationship between SIRT1 mRNA expression and related genes in PBMC. Notes: The expression level of the target mRNAs in PBMC was normalized to GAPDH expression level. All reactions were performed in duplicate. The correlation between SIRT1 and FOXO1, FOXO3, or p53 was calculated and the data are expressed by Spearman’s rank correlation coefficient. r is the correlation coefficient. Abbreviations: PBMC, peripheral blood mononuclear cell; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; FOXO, Forkhead box O.
Figure 3
Figure 3
The relationship between gene expressions in PBMC and sedentary or moderate physical activity level. Note: (A) SIRT1 mRNA, (B) FOXO1 mRNA, (C) FOXO3 mRNA, (D) p53 mRNA. Abbreviations: PBMC, peripheral blood mononuclear cell; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; FOXO, Forkhead box O.
Figure 3
Figure 3
The relationship between gene expressions in PBMC and sedentary or moderate physical activity level. Note: (A) SIRT1 mRNA, (B) FOXO1 mRNA, (C) FOXO3 mRNA, (D) p53 mRNA. Abbreviations: PBMC, peripheral blood mononuclear cell; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; FOXO, Forkhead box O.
Figure 4
Figure 4
Comparisons of each gene expression in PBMC according to GOLD stage. Notes: Comparisons of GOLD stage classes were performed by Student’s t-test. Bars shows mean and standard error of the mean. Abbreviations: GOLD, Global Initiative for chronic Obstructive Lung Disease; PBMC, peripheral blood mononuclear cell; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; FOXO, Forkhead box O.
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V体育安卓版 - References

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