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. 2017 Oct 27;7(1):14265.
doi: 10.1038/s41598-017-14539-x.

"V体育官网" Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach

Jiekai Yu  1 , Xiaohui Zhai  1   2 , Xiaofen Li  1 , Chenhan Zhong  1 , Cheng Guo  1 , Fuquan Yang  3 , Ying Yuan  4 , Shu Zheng  5   6
Affiliations

Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach

Jiekai Yu et al. Sci Rep. .

Abstract

Colorectal cancer (CRC) is a common malignant neoplasm worldwide. It is important to identify new biomarkers for the early detection of CRC. In this study, magnetic beads and the Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) platform were used to analyse CRC and healthy control (HC) serum samples. The CRC diagnosis pattern was established to have a specificity of 94. 7% and sensitivity of 92. 3% in a blind test. The candidate biomarker serine/threonine kinase 4 (STK4, also known as MST1) was identified by Tandem mass spectrometry (MS/MS) and verified with western blotting and enzyme-linked immunosorbent assay (ELISA). The results indicated that there was a higher concentration of MST1 in HC subjects than stage I CRC patients for the early detection of CRC and a lower concentration in stage IV patients than in other CRC patients. The sensitivity and specificity of MST1 combined with carcinoembryonic antigen (CEA) and faecal occult blood test (FOBT) in diagnosis of colorectal cancer were 92. 3% and 100%, respectively. Additionally, low MST1 expression was associated with the poor prognosis VSports手机版. These results illustrate that MST1 is a potential biomarker for early detection, prognosis and prediction of distant metastasis of CRC. .

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Biomarker SVM patterns. (a) Scatter diagram of the cross-validation results from the training set. (b) Scatter diagram of the results from the blind test set (Group 0 and 1 represent the HC subjects and CRC patients). (c) MST1 (2084 Da) expressed at low levels in the CRC group (group 1) in the training sets by MALDI-TOF-MS. (d) MST1 (2084 Da) expressed at low levels in the group CRC (group 1) in the test sets by MALDI-TOF-MS.
Figure 2
Figure 2
Purification and identification of MST1 (2084 Da). (a) Purification and detection data of the 2084 Da target biomarkers by MALDI-TOF-MS. (b) MS/MS spectra of K.GLEYLHFMR.K in the target biomarkers. (c) MS/MS spectra of R.AIFMIPTNPPPTFR in the target biomarkers.
Figure 3
Figure 3
Verification results of the MST1 protein. (a) Representative western blotting image of MST1 in the CRC and HC sera in one PVDF membrane. Full-length blots are presented in Supplementary Figure S2. (b) Scatterplot of the normalized western blotting intensity of MST1 in 20 CRC patients and 20 HCs. (c) Scatterplot of the MST1 ELISA concentrations in the HC samples and the CRC samples at different stages.
Figure 4
Figure 4
ROC curve of MST1 and CEA. (a) ROC curve for serum MST1 in CRC and HC. (b) ROC curve for serum CEA in CRC and HC. (c) ROC curve for serum MST1 in early stage CRC (stage I and II) and HC. (d) ROC curve for serum CEA in early stage CRC (stage I and II) and HC. (e) ROC curve for MST1 Combined with CEA and FOBT. (f) Kaplan–Meier survival analysis for MST1.
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VSports - References

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