<big dropzone="lZtKWEN"><noframes id="CCfGqT"> Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or VSports app下载. mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

Review
. 2017 Jun 24:5:22.
doi: 10.1186/s40364-017-0102-y. eCollection 2017.

Engineering CAR-T cells

Cheng Zhang  1 , Jun Liu  1 , Jiang F Zhong  2 , Xi Zhang  1
Affiliations
Review

Engineering CAR-T cells

Cheng Zhang et al. Biomark Res. .

Abstract

Chimeric antigen receptor redirected T cells (CAR-T cells) have achieved inspiring outcomes in patients with B cell malignancies, and are now being investigated in other hematologic malignancies and solid tumors VSports手机版. CAR-T cells are generated by the T cells from patients' or donors' blood. After the T cells are expanded and genetically modified, they are reinfused into the patients. However, many challenges still need to be resolved in order for this technology to gain widespread adoption. In this review, we first discuss the structure and evolution of chimeric antigen receptors. We then report on the tools used for production of CAR-T cells. Finally, we address the challenges posed by CAR-T cells. .

Keywords: CAR-T cells; Chimeric antigen receptor redirected T cells; Evolution; Production; Structure; Viral vector V体育安卓版. .

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Structure of chimeric antigen receptor (CAR). The CAR includes ectodomain, transmembrane domain and endodomain
Fig. 2
Fig. 2
Evolution of chimeric antigen receptor (CAR) from the first generation to the fourth generation. Single chain antibody (CD3ζ or FcεRIγ) links the ITAM at transmembrane region for the first generation. Costimulatory molecule (CM1), such as CD28, has been engineered to the signal transduction region for the second generation. Another costimulatory molecule (CM2) based on the second generation for the third generation has been engineered to the signal transduction region, such as combining CD134 or CD137. The interleukin-12 (IL-12) based on the second generation for the fourth generation has been engineered to the signal transduction region. ITAM: Immunoreceptor tyrosine-based activation motifs
See this image and copyright information in PMC

References

    1. Ramos CA, Dotti G. Chimeric antigen receptor (CAR)-engineered lymphocytes for cancer therapy. Expert Opin Biol Ther. 2011;11(7):855–873. doi: 10.1517/14712598.2011.573476. - DOI - PMC - PubMed
    1. Lipowska-Bhalla G, Gilham DE, Hawkins RE, Rothwell DG. Targeted immunotherapy of cancer with CAR T cells: achievements and challenges. Cancer Immunol Immunother. 2012;61(7):953–962. doi: 10.1007/s00262-012-1254-0. - DOI - PMC - PubMed
    1. Liu J, Zhong JF, Zhang X, Zhang C. Allogeneic CD19-CAR-T cell infusion after allogeneic hematopoietic stem cell transplantation in B cell malignancies. J Hematol Oncol. 2017;10(1):35. doi: 10.1186/s13045-017-0405-3. - DOI - PMC - PubMed
    1. Tasian SK, Gardner RA. CD19-redirected chimeric antigen receptor-modified T cells: a promising immunotherapy for children and adults with B-cell acute lymphoblastic leukemia (ALL) Ther Adv Hematol. 2015;6(5):228–241. doi: 10.1177/2040620715588916. - DOI - PMC - PubMed
    1. Gross G, Waks T, Eshhar Z. Expression of immunoglobulin-T-cell receptor chimeric molecules as functional receptors with antibody-type specificity. Proc Natl Acad Sci U S A. 1989;86(24):10024–10028. doi: 10.1073/pnas.86.24.10024. - "V体育官网入口" DOI - PMC - PubMed

Publication types