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. 2016 Aug 25:7:1212.
doi: 10.3389/fpls.2016.01212. eCollection 2016.

"VSports" Fluorescent Pseudomonas Strains with only Few Plant-Beneficial Properties Are Favored in the Maize Rhizosphere

Jordan Vacheron  1 , Yvan Moënne-Loccoz  1 , Audrey Dubost  1 , Maximilien Gonçalves-Martins  1 , Daniel Muller  1 , Claire Prigent-Combaret  1
Affiliations

Fluorescent Pseudomonas Strains with only Few Plant-Beneficial Properties Are Favored in the Maize Rhizosphere

Jordan Vacheron et al. Front Plant Sci. .

Abstract (V体育2025版)

Plant Growth-Promoting Rhizobacteria (PGPR) enhance plant health and growth using a variety of traits. Effective PGPR strains typically exhibit multiple plant-beneficial properties, but whether they are better adapted to the rhizosphere than PGPR strains with fewer plant-beneficial properties is unknown VSports手机版. Here, we tested the hypothesis that strains with higher numbers of plant-beneficial properties would be preferentially selected by plant roots. To this end, the co-occurrence of 18 properties involved in enhanced plant nutrition, plant hormone modulation, or pathogen inhibition was analyzed by molecular and biochemical methods in a collection of maize rhizosphere and bulk soil isolates of fluorescent Pseudomonas. Twelve plant-beneficial properties were found among the 698 isolates. Contrarily to expectation, maize preferentially selected pseudomonads with low numbers of plant-beneficial properties (up to five). This selection was not due to the predominance of strains with specific assortments of these properties, or with specific taxonomic status. Therefore, the occurrence of only few plant-beneficial properties appeared favorable for root colonization by pseudomonads. .

Keywords: PGPR; fluorescent Pseudomonas; functional groups; maize; plant-beneficial properties V体育安卓版. .

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"V体育ios版" Figures

FIGURE 1
FIGURE 1
Proportion of pseudomonads according to the type of plant-beneficial properties harbored, in bulk soil and in DK315 and PR37Y15 maize rhizospheres. Data from soils Bmo1, Ysa5, MS8, and Ysa8 were combined and error bars (standard errors) reflect the variability among the four soils. HCN, hydrogen cyanide; DAPG, 2,4-diacetylphloroglucinol; ACC, 1-amino-cyclopropane carboxylic acid; NO, nitric oxide.
FIGURE 2
FIGURE 2
Prevalence of plant-beneficial properties in pseudomonads in bulk soil and in DK315 and PR37Y15 maize rhizospheres at 21 days in soils Bmo1, Ysa5, MS8, and Ysa8. The heatmap represents the proportion of fluorescent pseudomonads harboring each plant-beneficial property amongst the 12 targeted and detected ones. Column clustering was performed using the correlation method. B, Bulk soil (brown rounded rectangles); PR, cultivar PR37Y15 (green rounded rectangles); DK, cultivar DK315 (gray rounded rectangles).
FIGURE 3
FIGURE 3
Proportion (A) and estimated number (B) of pseudomonads according to the number of co-occurring plant-beneficial properties harbored (from 0 to 9), in bulk soil and in DK315 and PR37Y15 maize rhizospheres. Data from soils Bmo1, Ysa5, MS8, and Ysa8 were combined and error bars (standard errors) reflect the variability among the four soils. The number of Pseudomonas was estimated by multiplying the Pseudomonas proportions with the population levels quantified in each bulk and rhizosphere soil. Statistical differences between conditions (bulk soil/PR37Y15/DK315) are indicated with letter a-b (ANOVA and Fisher’s LSD tests. P < 0.05).
FIGURE 4
FIGURE 4
Distribution of plant-beneficial properties in Pseudomonas isolates according to (A) their taxonomic affiliation and (B) number of co-occurring plant-beneficial properties per isolate among the 18 plant-beneficial properties analyzed. The topology of the cladogram has been drawn according to the phylogenetic classification of Gomila et al. (2015). Both clusters are indicated. Biocontrol properties include production of antimicrobial compounds such as DAPG, pyrrolnitrin, pyoluteorin, phenazine, and HCN, and production of extracellular protease and chitinase. Plant hormonal and nutrition properties regroup ACC deaminase, production of NO, kinetin and IAA and phosphate solubilization.
FIGURE 5
FIGURE 5
Comparison of the proportion of pseudomonads belonging to FMJK and CPC clusters and to P. putida group in bulk soil and in the PR37Y15 and DK315 maize rhizospheres. Error bars correspond to standard errors. Statistical differences between subgroups are indicated with letter a-b and statistical differences between conditions (bulk soil/PR37Y15/ DK315) are indicated with letter α-β (ANOVA and Fisher’s LSD tests. P < 0.05).
FIGURE 6
FIGURE 6
Comparison of the proportion of pseudomonads belonging to FMJK (A) and CPC (B) clusters in bulk soil and in the PR37Y15 and DK315 maize rhizospheres according to the number of co-occurring plant-beneficial properties harbored. Error bars correspond to standard errors. Statistical differences between plant-beneficial property classes according to conditions (bulk soil/PR37Y15/DK315) are indicated with letter α-β (ANOVA and Fisher’s LSD tests. P < 0.05). Statistical differences between conditions within plant-beneficial property class are indicated with letter a-b.
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