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. 2016 Aug 15;35(16):1766-78.
doi: 10.15252/embj.201694696. Epub 2016 Jul 14.

GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death

Lorenzo Sborgi  1 , Sebastian Rühl  1 , Estefania Mulvihill  2 , Joka Pipercevic  1 , Rosalie Heilig  1 , Henning Stahlberg  1 , Christopher J Farady  3 , Daniel J Müller  2 , Petr Broz  4 , Sebastian Hiller  4
Affiliations

GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death

"V体育官网" Lorenzo Sborgi et al. EMBO J. .

Abstract

Pyroptosis is a lytic type of cell death that is initiated by inflammatory caspases VSports手机版. These caspases are activated within multi-protein inflammasome complexes that assemble in response to pathogens and endogenous danger signals. Pyroptotic cell death has been proposed to proceed via the formation of a plasma membrane pore, but the underlying molecular mechanism has remained unclear. Recently, gasdermin D (GSDMD), a member of the ill-characterized gasdermin protein family, was identified as a caspase substrate and an essential mediator of pyroptosis. GSDMD is thus a candidate for pyroptotic pore formation. Here, we characterize GSDMD function in live cells and in vitro We show that the N-terminal fragment of caspase-1-cleaved GSDMD rapidly targets the membrane fraction of macrophages and that it induces the formation of a plasma membrane pore. In vitro, the N-terminal fragment of caspase-1-cleaved recombinant GSDMD tightly binds liposomes and forms large permeability pores. Visualization of liposome-inserted GSDMD at nanometer resolution by cryo-electron and atomic force microscopy shows circular pores with variable ring diameters around 20 nm. Overall, these data demonstrate that GSDMD is the direct and final executor of pyroptotic cell death. .

Keywords: atomic force microscopy; cell death; gasdermin; inflammasomes; inflammation; pyroptosis V体育安卓版. .

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V体育2025版 - Figures

Figure 1
Figure 1. GSDMD Nterm induces the formation of a large plasma membrane pore
  1. A

    Cell viability as assessed by GFP expression in HEK293T cells transfected with the pRetroX TetOne3G‐eGFP plasmid only (vector) or pRetroX TetOne3G‐eGFP harboring the N‐terminal fragment of GSDMD. Cells were treated with the indicated concentrations of doxycycline 24 h post‐transfection, and the percentage of GFP‐positive cells was determined 16 h later by flow cytometry V体育平台登录.

  2. B

    LDH release from HEK293T cells transfected with the pRetroX TetOne3G‐eGFP plasmid only (vector) or pRetroX TetOne3G‐eGFP harboring the N‐terminal fragment of GSDMD. At 24 h post‐transfection, cells were treated with the indicated concentrations of doxycycline for 8 h and the percentage of LDH release was determined VSports注册入口. Graphs show mean and s. d. of quadruplicate wells.

  3. C, D

    PI staining of and LDH release from HEK293T cells transfected with pRetroX TetOne3G‐eGFP harboring the N‐terminal fragment of GSDMD in the presence of osmoprotectants. At 24 h post‐transfection, PEGs of the indicated molecular weights were added to a final concentration of 30 mM, cells were treated with 250 ng ml−1 doxycycline for 8 h, and the level of PI staining (C) or LDH release (D) was determined V体育官网入口.

  4. E, F

    PI staining of and LDH release from LPS‐primed primary BMDMs infected with log‐phase S VSports在线直播.  typhimurium for the indicated time points in the presence of PEGs of the indicated molecular weight (numbers on the x‐axis, 30 mM final concentration).

Data information: Graphs show mean and s.d. of quadruplicate wells (B–F) or the mean and s.d. of duplicate wells (A). *P < 0.05 as determined by Student's t‐test. Data are representative of at least three independent experiments.
Figure 2
Figure 2. GSDMD Nterm localizes to cellular membranes after inflammasome activation

  1. Immunoblot analysis of cleaved GSDMD in culture supernatants and full‐length GSDMD, cleaved GSDMD and α‐tubulin in the cell lysates of immortalized LPS‐primed WT macrophages left uninfected (NS) or infected for 10–40 min with log‐phase S. typhimurium (MOI = 50).

  2. Schematic representation of the subcellular fractionation shown in (C).

  3. Fractionation and immunoblot analysis for GSDMD, Na+K+ ATPase, VDAC (voltage‐dependent anion channel), HDAC1 (histone deacetylase 1), and GAPDH (glycerinaldehyd‐3‐phosphate dehydrogenase) of WT macrophages infected for 10 and 20 min with log‐phase S. typhimurium (MOI = 50). Fractionation was carried out as described in the Materials and Methods section, and equivalent amount of protein was loaded per lane.

  4. Extraction of cleaved GSDMD from isolated membranes of WT macrophages infected for 10 min with log‐phase S. typhimurium (MOI = 50). Extraction was carried in variable conditions as described in the Materials and Methods section.

Source data are available online for this figure.
Figure 3
Figure 3. GSDMD Nterm targets liposomes after caspase‐1 cleavage

  1. Human GSDMD at a concentration of 2 μM was incubated at room temperature with 5 nM caspase‐1. The protein was cleaved in a time‐dependent manner into two bands of 31 kDa (GSDMD Nterm) and 22 kDa (GSDMDCterm).

  2. Cross‐linking experiment of full‐length and cleaved GSDMD. GSDMD at a concentration of 2 μM was incubated at room temperature with 5 nM caspase‐1. After enzymatic cleavage, GSDMDNterm is highly cross‐linked by DSS, resulting in the gel‐impenetrating species highlighted by the arrow. GSDMDCterm is not cross‐linked.

  3. GSDMD at a concentration of 1 μM was incubated at room temperature with 5 nM caspase‐1 and liposomes composed of 4 mM DMPC or polar lipid extract derived from E. coli. After 2 h, the lipid fraction (L) was separated from the supernatant (S) by ultracentrifugation at 4°C for 1 h at 120,000 g.

Source data are available online for this figure.
Figure 4 "VSports最新版本"
Figure 4. GSDMD Nterm causes liposome permeability by pore formation
  1. A–I

    Dye release time courses from liposomes as a percentage of maximal release. (A) Five different reactions, where 5 nM caspase‐1 and 400 μM 6‐carboxyfluorescein‐loaded liposomes prepared with E. coli polar lipids were incubated with GSDMD concentrations of (nM): 520, 260, 130, 100, 65 (colored dark to light orange). The time point of 20 min is highlighted by a vertical dashed line. (B) Five different reactions, where 130 nM of GSDMD and 400 μM 6‐carboxyfluorescein‐loaded liposomes prepared with E. coli extract polar lipid, were incubated with caspase‐1 concentration of (nM): 15, 8, 5, 2.5, 1.2 (colored dark to light blue). The time point of 20 min is highlighted by a vertical dashed line. (C) Three different reactions, where 5 nM caspase‐1 and 400 μM 6‐carboxyfluorescein‐loaded liposomes prepared with porcine brain total lipid extract, were incubated with GSDMD concentrations of (nM): 520, 260, 100 (colored dark to light green). (D) Dye release at 20‐min reaction as a function of GSDMD (dark orange) and caspase‐1 (dark blue) concentrations. Error bars for three independent experiments are shown. (E) Two different sets of reactions, where wild‐type GSDMD (dark to light orange) and the mutant GSDMDI104N (dark to light blue) were independently incubated at the concentrations of 260, 130, and 65 nM with 5 nM caspase‐1 and 400 μM 6‐carboxyfluorescein‐loaded liposomes. The time point of 60 min is highlighted by a vertical dashed line. (F) Dye release at 60 min of reaction as a function of GSDMD wild‐type (dark orange) and GSDMDI104N (dark blue) concentration. Error bars for three independent experiments are shown. (G–I) Dye release from 400 μM liposomes loaded with the 6‐carboxyfluorescein derivates FD‐20, FD‐40, and FD‐150, with variable Stokes diameters, as indicated. 130 nM of GSDMD and 5 nM caspase‐1 were incubated with the liposomes. For each experiment, a representative from three independent experiments is shown. The corresponding raw data are shown in Appendix Figure S5.

Figure 5
Figure 5. Visualization of GSDMD Nterm pores in liposomes by cryo‐electron microscopy
  1. A–C

    Cryo‐electron micrographs of GSDMDNterm pores in E. coli polar lipid liposomes. The micrographs were acquired at protein/lipid molar ratios of 1/1,000, 1/500, and 1/100, respectively. Black arrows indicate ring‐shaped structures corresponding to oligomeric GSDMDNterm pore forms. Scale bars = 80 nm.

  2. D

    Proteoliposome with protein/lipid molar ratio of 1/100 at higher magnification. Black arrows indicate ring‐shaped structures corresponding to oligomeric GSDMDNterm pore forms. Scale bar = 80 nm.

Figure 6
Figure 6. Characterization of GSDMD Nterm pores by atomic force microscopy
  1. A

    AFM topograph of GSDMDNterm bound to lipid membranes of E. coli polar lipids. Overview topograph showing arc‐, slit‐, and ring‐like GSDMD oligomers. Scale bar corresponds to 100 nm.

  2. B–E

    High‐resolution AFM topographs and height profiles of GSDMDNterm oligomers (red curves). Height profiles were measured along the red lines in the topographs. Dashed zero lines (0 nm) indicate the surface of the lipid membrane; heights of < −4 nm indicate that the oligomers formed transmembrane pores. Topographs were taken in buffer solution (150 NaCl, 20 mM Hepes, pH 7.8) at room temperature. The experiment was reproduced at least three times using independent liposome and GSDMD preparations. Scale bars of topographs correspond to 50 nm and of height profiles 10 nm. The full color range of the AFM topographs corresponds to a vertical scale (height) of 20 nm.

  3. F–H

    Analysis of GSDMDNterm oligomers bound to lipid membranes of E. coli polar lipids. (F) GSDMDNterm oligomers assembled into arcs, slits, and rings leading to transmembrane (TM) pores or not transmembrane aggregate (n = 218). (G) Height of GSDMDNterm oligomers protruding from the lipid membrane. Bars represent mean, and error bars represent s.e.m. (n = 218). (H) Distribution of the diameters of rings formed by GSDMDNterm oligomers. The average distribution was 21.2 ± 5.6 nm (n = 164; average ± s.d.), and the bin size was 3 nm.

Figure 7
Figure 7. Model for GSDMD‐mediated pyroptotic cell death
Canonical inflammasomes act as sensors for a variety of pathogens and cellular insults. Assembly of these types of inflammasomes involves multimerization of the receptor (purple), which initiates filament formation of the adaptor protein ASC (green). ASC filaments act as activation platforms for caspase‐1 (blue). In the noncanonical pathway, LPS is directly bound by caspase‐11 (yellow), resulting in its activation. Caspase‐1 processes interleukin IL‐1β (red). Both caspase‐1 and caspase‐11 process GSDMD (orange), which results in release of the GSDMDNterm fragment. GSDMDNterm forms a large pore in the plasma membrane. Pore formation results in rapid loss of membrane integrity, the dissipation of the electrochemical gradient, and ultimately in cell death.
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