Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. VSports app下载.

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 2015 Nov 2:111:7.23.1-7.23.16.
doi: 10.1002/0471142735.im0723s111.

Isolation and Functional Analysis of Human Neutrophils

Douglas B Kuhns  1 , Debra A Long Priel  1 , Jessica Chu  2 , Kol A Zarember  2
Affiliations

Isolation and Functional Analysis of Human Neutrophils

Douglas B Kuhns et al. Curr Protoc Immunol. .

Abstract

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described VSports手机版. .

Keywords: NOX2; innate immunity; leukocyte; neutrophil; phagocyte V体育安卓版. .

PubMed Disclaimer

"V体育2025版" References

    1. Haslett C, Guthrie LA, Kopaniak MM, Johnston RB, Jr, Henson PM. Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide. Am J Pathol. 1985;119:101–110. - PMC - PubMed
    1. Nathan C. Neutrophils and immunity: challenges and opportunities. Nat Rev Immunol. 2006;6:173–182. - PubMed
    1. Nauseef WM. How human neutrophils kill and degrade microbes: an integrated view. Immunol Rev. 2007;219:88–102. - "VSports注册入口" PubMed
    1. Parker LC, Prince LR, Buttle DJ, Sabroe I. The generation of highly purified primary human neutrophils and assessment of apoptosis in response to Toll-like receptor ligands. Methods Mol Biol. 2009;517:191–204. - PubMed
    1. Quinn MT, Deleo F. Neutrophil methods and protocols. (Second edition) - PubMed

Publication types

MeSH terms