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. 2015 Oct;25(10):1499-507.
doi: 10.1101/gr.191098.115.

The first five years of single-cell cancer genomics and beyond

Nicholas E Navin  1
Affiliations

The first five years of single-cell cancer genomics and beyond

Nicholas E Navin. Genome Res. 2015 Oct.

Abstract

Single-cell sequencing (SCS) is a powerful new tool for investigating evolution and diversity in cancer and understanding the role of rare cells in tumor progression VSports手机版. These methods have begun to unravel key questions in cancer biology that have been difficult to address with bulk tumor measurements. Over the past five years, there has been extraordinary progress in technological developments and research applications, but these efforts represent only the tip of the iceberg. In the coming years, SCS will greatly improve our understanding of invasion, metastasis, and therapy resistance during cancer progression. These tools will also have direct translational applications in the clinic, in areas such as early detection, noninvasive monitoring, and guiding targeted therapy. In this perspective, I discuss the progress that has been made and the myriad of unexplored applications that still lie ahead in cancer research and medicine. .

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Figures

Figure 1.
Figure 1.
Timeline of single-cell sequencing milestones in cancer research. This timeline depicts the major discoveries that have occurred in the cancer field of single-cell sequencing and related technology developments.
Figure 2.
Figure 2.
Applications of single-cell sequencing in cancer research. This figure displays the myriad of applications that single-cell sequencing methods have in cancer research: (A) resolving intratumor heterogeneity; (B) investigating clonal evolution in primary tumors; (C) studying invasion in early stage cancers; (D) tracing metastatic dissemination; (E) genomic profiling of circulating tumor cells; (F) investigating mutation rates and mutator phenotypes; (G) understanding resistance evolution to therapy; (H) understanding cancer stem cells and cell hierarchies; and (I) studying cell plasticity and epithelial-to-mesenchymal transition.
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