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Observational Study
. 2015 Nov;12(11):1662-6.
doi: 10.1513/AnnalsATS.201505-267BC.

VSports注册入口 - Pyruvate Dehydrogenase Activity Is Decreased in the Peripheral Blood Mononuclear Cells of Patients with Sepsis. A Prospective Observational Trial

Erin Nuzzo  1 , Katherine M Berg  2 , Lars W Andersen  3   4 , Julia Balkema  3 , Sophia Montissol  3 , Michael N Cocchi  3   5 , Xiaowen Liu  3 , Michael W Donnino  2   3
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Observational Study

Pyruvate Dehydrogenase Activity Is Decreased in the Peripheral Blood Mononuclear Cells of Patients with Sepsis. A Prospective Observational Trial

Erin Nuzzo et al. Ann Am Thorac Soc. 2015 Nov.

"VSports注册入口" Abstract

Rationale: Rodent studies have shown that pyruvate dehydrogenase (PDH) levels are low in sepsis. This may cause cells to shift to anaerobic metabolism, resulting in increased lactate production. Alterations in PDH during sepsis have never been studied in humans VSports手机版. .

Objectives: The objective of this pilot study was to measure PDH activity and quantity in patients with severe sepsis V体育安卓版. .

Methods: We conducted a pilot case-control study at a single urban tertiary care center. We compared PDH activity and quantity between patients with severe sepsis admitted to the intensive care unit and healthy control subjects V体育ios版. PDH activity and quantity were measured in isolated peripheral blood mononuclear cells. We measured PDH activity and quantity in control subjects at baseline and in patients with sepsis at 0 (baseline), 24, 48, and 72 hours. .

Measurements and main results: We enrolled 56 patients with sepsis and 20 control subjects with at least one blood sample being drawn from each patient. PDH activity and quantity in the sepsis group were significantly lower than the control group (P < 0. 001). In multivariable linear regression adjusting for age, race, sex, and assay plate, the difference remained significant. Patients with sepsis who died had significantly lower PDH activity compared with those who survived (P = 0 VSports最新版本. 03). .

Conclusions: PDH activity and quantity is decreased in peripheral blood mononuclear cells of humans with severe sepsis when compared with healthy control subjects, and may be associated with mortality V体育平台登录. Whether decreased PDH activity plays a role in lactate metabolism or whether pharmacologic modification of PDH activity may improve outcomes remains unknown. .

Keywords: aerobic; lactate; metabolism; mitochondria; mortality VSports注册入口. .

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Figures

Figure 1.
Figure 1.
Pyruvate dehydrogenase (PDH) values in patients with sepsis and control subjects. PDH activity (A), quantity (B), and specific activity (C) in control subjects and patients with sepsis at enrollment (0 hours) and 24, 48, and 72 hours thereafter. Compared with baseline values in the septic cohort, control subjects had significantly higher activity, quantity, and specific activity.
Figure 2.
Figure 2.
Pyruvate dehydrogenase (PDH) activity in survivors and nonsurvivors. PDH activity in the septic cohort stratified by survival status. Patients who survived had significantly higher PDH activity over time as compared with those not surviving (P = 0.02).
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References

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