Motivation: Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data. VSports手机版.

Results: A5-miseq can produce high-quality microbial genome assemblies on a laptop computer without any parameter tuning. A5-miseq does this by automating the process of adapter trimming, quality filtering, error correction, contig and scaffold generation and detection of misassemblies. Unlike the original A5 pipeline, A5-miseq can use long reads from the Illumina MiSeq, use read pairing information during contig generation and includes several improvements to read trimming. Together, these changes result in substantially improved assemblies that recover a more complete set of reference genes than previous methods. V体育安卓版.

Availability: A5-miseq is licensed under the GPL open-source license. Source code and precompiled binaries for Mac OS X 10. 6+ and Linux 2. 6. 15+ are available from http://sourceforge. net/projects/ngopt V体育ios版.

Contact: aaron.darling@qiuluzeuv.cn

Supplementary information: Supplementary data are available at Bioinformatics online V体育平台登录. .