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Comparative Study
. 2014 Feb 14;9(2):e88319.
doi: 10.1371/journal.pone.0088319. eCollection 2014.

Comparison of angiogenic, cytoprotective, and immunosuppressive properties of human amnion- and chorion-derived mesenchymal stem cells

Kenichi Yamahara  1 , Kazuhiko Harada  2 , Makiko Ohshima  1 , Shin Ishikane  2 , Shunsuke Ohnishi  3 , Hidetoshi Tsuda  1 , Kentaro Otani  1 , Akihiko Taguchi  4 , Toshihiro Soma  5 , Hiroyasu Ogawa  5 , Shinji Katsuragi  6 , Jun Yoshimatsu  6 , Mariko Harada-Shiba  7 , Kenji Kangawa  2 , Tomoaki Ikeda  8
Affiliations
Comparative Study

Comparison of angiogenic, cytoprotective, and immunosuppressive properties of human amnion- and chorion-derived mesenchymal stem cells

VSports在线直播 - Kenichi Yamahara et al. PLoS One. .

Abstract

Although mesenchymal stem cells (MSCs) can be obtained from the fetal membrane (FM), little information is available regarding biological differences in MSCs derived from different layers of the FM or their therapeutic potential. Isolated MSCs from both amnion and chorion layers of FM showed similar morphological appearance, multipotency, and cell-surface antigen expression. Conditioned media obtained from amnion- and chorion-derived MSCs inhibited cell death caused by serum starvation or hypoxia in endothelial cells and cardiomyocytes. Amnion and chorion MSCs secreted significant amounts of angiogenic factors including HGF, IGF-1, VEGF, and bFGF, although differences in the cellular expression profile of these soluble factors were observed. Transplantation of human amnion or chorion MSCs significantly increased blood flow and capillary density in a murine hindlimb ischemia model. In addition, compared to human chorion MSCs, human amnion MSCs markedly reduced T-lymphocyte proliferation with the enhanced secretion of PGE2, and improved the pathological situation of a mouse model of acute graft-versus-host disease. Our results highlight that human amnion- and chorion-derived MSCs, which showed differences in their soluble factor secretion and angiogenic/immuno-suppressive function, could be ideal cell sources for regenerative medicine VSports手机版. .

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Characterization of human amnion- and chorion-derived MSCs.
(A) Representative photographs of human amnion and chorion. (B) Photographs of cultured MSCs obtained from human amnion and chorion at passage 3. Scale bars = 500 µm. (C) Relative cell number of amnion- and chorion-derived MSCs at each passage. (D) FACS analysis of amnion and chorion MSCs. (E, F) Differentiation of amnion and chorion MSCs into adipocytes (E) and osteocytes (F). Scale bars = 100 (E) and 50 (F) µm.
Figure 2
Figure 2. Growth factor secretion and the cytoprotective effect of amnion and chorion MSCs.
(A–D) Cytoprotective effect of FM MSC-derived conditioned medium was analyzed by the MTS assay (A, B) and caspase-3 activity (C, D) in HUVECs (A, C) and cardiomyocytes (B, D). Values are mean ± SEM. *p<0.05 vs. serum-free. (E–H) Conditioned medium obtained from FM-derived MSCs was collected after incubation for 24 h. The concentration of HGF (E), IGF-1 (F), bFGF (G), and VEGF (H) in serum free conditioned medium was measured by ELISA. *p<0.05 and ***p<0.001.
Figure 3
Figure 3. Angiogenic potential of amnion and chorion MSCs against hindlimb ischemia.
(A) Representative images of serial hindlimb blood perfusion. Blood perfusion of ischemic hindlimb increased in the amnion and chorion MSC groups at day 5. (B) Quantitative analysis of hindlimb blood perfusion with the LDPI index, the ratio of ischemic to non-ischemic hindlimb blood perfusion. (C) Representative photographs of immunohistochemistry with anti-CD31 antibody. Scale bars = 100 µm. (D) Quantitative analysis of capillary density in ischemic hindlimb muscle at day 5 among the control, amnion, and chorion MSC groups. Capillary density is shown as the capillary-to-muscle-fiber ratio. Data are mean ± SEM. **p<0.01 and ***p<0.001 vs. control.
Figure 4
Figure 4. Immunosuppressive property of amnion and chorion MSCs.
(A) Inhibition of human CD4+ T cell proliferation upon co-culture with human amnion, chorion, and bone marrow MSCs. (B) The concentration of PGE2 in FM-MSC-conditioned medium was measured by ELISA. Amnion MSCs secreted a significant amount of PGE2 compared with chorion MSCs. (C, D) Effect of human amnion (C) or chorion (D) MSC transplantation in a murine GVHD model. Treatment with amnion MSCs significantly reduced recipient weight loss in a mouse model of GVHD. *p<0.05, **p<0.01 and ***p<0.001.
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References

    1. Prockop DJ (1997) Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276: 71–74. - PubMed (VSports app下载)
    1. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, et al. (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13: 4279–4295. - PMC (V体育ios版) - PubMed
    1. Ishikane S, Ohnishi S, Yamahara K, Sada M, Harada K, et al. (2008) Allogeneic injection of fetal membrane-derived mesenchymal stem cells induces therapeutic angiogenesis in a rat model of hind limb ischemia. Stem Cells 26: 2625–2633. - PubMed
    1. Ishikane S, Yamahara K, Sada M, Harada K, Kodama M, et al. (2010) Allogeneic administration of fetal membrane-derived mesenchymal stem cells attenuates acute myocarditis in rats. J Mol Cell Cardiol 49: 753–761. - PubMed
    1. Tsuda H, Yamahara K, Ishikane S, Otani K, Nakamura A, et al. (2010) Allogenic fetal membrane-derived mesenchymal stem cells contribute to renal repair in experimental glomerulonephritis. Am J Physiol Renal Physiol 299: F1004–1013. - PubMed

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