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. 2014 Feb;11(2):163-6.
doi: 10.1038/nmeth.2772. Epub 2013 Dec 22.

"V体育2025版" Quantitative single-cell RNA-seq with unique molecular identifiers

Saiful Islam  1 , Amit Zeisel  1 , Simon Joost  2 , Gioele La Manno  1 , Pawel Zajac  1 , Maria Kasper  2 , Peter Lönnerberg  1 , Sten Linnarsson  1
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"VSports在线直播" Quantitative single-cell RNA-seq with unique molecular identifiers

Saiful Islam et al. Nat Methods. 2014 Feb.

Abstract (VSports app下载)

Single-cell RNA sequencing (RNA-seq) is a powerful tool to reveal cellular heterogeneity, discover new cell types and characterize tumor microevolution. However, losses in cDNA synthesis and bias in cDNA amplification lead to severe quantitative errors. We show that molecular labels--random sequences that label individual molecules--can nearly eliminate amplification noise, and that microfluidic sample preparation and optimized reagents produce a fivefold improvement in mRNA capture efficiency VSports手机版. .

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References

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    1. Nat Protoc. 2009;4(1):44-57 - PubMed
    1. Nat Methods. 2013 Nov;10(11):1096-8 - PubMed (V体育ios版)
    1. Stem Cells. 2013 Jan;31(1):48-58 - PubMed
    1. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17454-9 - PubMed

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