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. 2013 Jun 20;8(6):e66074.
doi: 10.1371/journal.pone.0066074. Print 2013.

Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform

Tore Midtvedt  1 , Eugene Zabarovsky, Elisabeth Norin, Johan Bark, Rinat Gizatullin, Vladimir Kashuba, Olle Ljungqvist, Veronika Zabarovska, Roland Möllby, Ingemar Ernberg
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Increase of faecal tryptic activity relates to changes in the intestinal microbiome: analysis of Crohn's disease with a multidisciplinary platform

Tore Midtvedt et al. PLoS One. .

Abstract

Objective: To investigate-by molecular, classical and functional methods-the microbiota in biopsies and faeces from patients with active Crohn's disease (CD) and controls VSports手机版. .

Design: The microbiota in biopsies was investigated utilizing a novel molecular method and classical cultivation technology V体育安卓版. Faecal samples were investigated by classical technology and four functional methods, reflecting alterations in short chain fatty acids pattern, conversion of cholesterol and bilirubin and inactivation of trypsin. .

Results: By molecular methods we found more than 92% similarity in the microbiota on the biopsies from the two groups. However, 4 V体育ios版. 6% of microbes found in controls were lacking in CD patients. Furthermore, NotI representation libraries demonstrate two different clusters representing CD patients and controls, respectively. Utilizing conventional technology, Bacteroides (alt. Parabacteroides) was less frequently detected in the biopsies from CD patients than from controls. A similar reduction in the number of Bacteroides was found in faecal samples. Bacteroides is the only group of bacteria known to be able to inactivate pancreatic trypsin. Faecal tryptic activity was high in CD patients, and inversely correlated to the levels of Bacteroides. .

Conclusions: CD patients have compositional and functional alterations in their intestinal microbiota, in line with the global description hypothesis rather than the candidate microorganism theory. The most striking functional difference was high amount of faecal tryptic activity in CD patients, inversely correlated to the levels of Bacteroides in faeces. VSports最新版本.

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Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Hybridization of control and CD patients “NotI representation” libraries to oligonucleotide-probes in biopsies.
Numbers reflect strength of signal after hybridization. Corresponding bacterial names are shown in the bottom row. Ruminococcus flavefaciens demonstrating the largest difference in the signal strength is shown in grey. The signals were clustered into a dendrogram using Euclidean distances depicted to the left. Crohn patients (C1–C4); controls (H1–H5).
Figure 2
Figure 2. Bacterial DNA unique to Crohn patients (C) or controls (H) as demonstrated by PCR-amplification using primers according to table 2.
PCR group 1 bacterial DNA was found in all four controls, while groups 3–5 and VPI were only detected in biopsy samples from the Crohn patients. By sequencing of the PCR-products (not shown) it could be shown that groups 1–5 represent bacteria not previously identified, while VPI represents Bacteroides thetaiotaomicron VPI-5482.
Figure 3
Figure 3. Cfu∶s of Bacteroides spp. and of E. coli per biopsy or per gram faeces.
CD patients versus controls. Horisontal line depicts mean number of log cfu∶s ( = geometric mean of cfu∶s) and the upper T depicts SEM. Note that three out of five biopsies from CD patients did not show any growth of Bacteroides, while all control biopsies did (p = 0.054, Mann-Whitney).
Figure 4
Figure 4. Levels of three MAC∶s in faecal samples from Crohn patients versus healthy controls.
Horizontal line depicts mean value.
Figure 5
Figure 5. Relation between log nr of Bacteroides in faeces and faecal tryptic activity (FTA).
Numbers denote FTA activity, p-value = 0.0011 (Person). It is to be noted that all control samples had high numbers of Bacteroides and low levels of FTA (Figures 3 and 4). *denotes a CD patient with ileo-caecal resection, why he was not included in other data in this manuscript. One CD patient was not included since his faecal sample was not subjected to cultivation for technical reasons.
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References

    1. Dumonceau JM, Adler M, Cremer M, Van Gossum A (1994) The role of infectious agents in Crohn's disease. Acta Gastroenterol Belg 57 (5–6) 301–5. - PubMed
    1. Marteau P, Lepage P, Mangin I, Suau A, Doré J, et al. (2004) Review article: gut flora and inflammatory bowel disease. Aliment Pharmacol Ther Suppl 4: 18–23. - PubMed
    1. World Health Organization (1997) Implementation of the WHO Global Strategy for Occupational Health for All. Plan of action: covering the specific period 1996–2001. Part 1 Int J Occup Med Environ Health 10 (2) 113–39. - PubMed
    1. Bergstrand LO, Gustafsson BE, Holmstrom B, Norin KE (1981) The physiological activity of human ileal flora in patients with Crohn's disease and ulcerative colitis evaluated by determination of germfree animal characteristics. Acta Chir Scand 147: 707–9. - "VSports" PubMed
    1. van der Merve JP, Mol GJJ (1982) Levels of trypsin and alpha-chymotrypsin in feces from patients with Crohn's disease. Digestion 24: 1–4. - PubMed

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