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. 2013 Sep 28;338(2):267-70.
doi: 10.1016/j.canlet.2013.04.001. Epub 2013 Apr 11.

"V体育ios版" Pyrimidine base damage is increased in women with BRCA mutations

Edwin E Budzinski  1 , Helen B Patrzyc, Jean B Dawidzik, Harold G Freund, Peter Frederick, Heidi E Godoy, Nicoleta C Voian, Kunle Odunsi, Harold C Box
Affiliations

Pyrimidine base damage is increased in women with BRCA mutations (VSports注册入口)

Edwin E Budzinski et al. Cancer Lett. .

"VSports手机版" Abstract

Oxidatively-induced DNA damage was measured in the DNA of WBC from two groups of women: carriers of a BRCA mutation, but asymptomatic for disease, and healthy controls. Two oxidatively induced lesions were measured: a formamide remnant of pyrimidine base and the glycol modification of thymine. These lesions, employed previously in studies of the effects of smoking, antioxidant usage and ovarian cancer, are proving valuable indicators of oxidative stress. The BRCA carriers of mutations, with no overt sign of cancer, nevertheless had significantly higher levels of DNA damage than the controls. The level measured for the formamide lesion was 5. 9 ± 1. 0 (femtomoles/μg of DNA ± SEM) compared with 2 VSports手机版. 4 ± 0. 3 in controls. The level of the glycol lesion was 2. 9 ± 0. 4 compared with 1. 8 ± 0. 2 in controls. The experimental design utilized DNA from WBC and employed LC-MS/MS to detect the lesions. .

Keywords: BRCA mutations; Glycol lesion; Ovarian cancer; Oxidative DNA damage. V体育安卓版.

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Conflict of interest statement

Conflict of Interest Statement

None

Figures (V体育2025版)

Fig. 1
Fig. 1
The formamide and thymine glycol lesions shown incorporated in dinucleoside monophosphates, the form in which they are actually measured.
Fig. 2
Fig. 2
Levels of d(PfpA) (fmol/ug DNA) versus age in carriers of BRCA mutations (empty circles) and healthy controls (filled circles).
Fig. 3
Fig. 3
Levels of d(TgpA) (fmol/ug DNA) versus age in carriers of BRCA mutations (empty circles) and healthy controls (filled circles).
See this image and copyright information in PMC

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