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. 2013 Apr 8:13:61.
doi: 10.1186/1471-230X-13-61.

Possible role of Escherichia coli in propagation and perpetuation of chronic inflammation in ulcerative colitis

Magdalena Pilarczyk-Zurek  1 , Agnieszka Chmielarczyk, Tomasz Gosiewski, Anna Tomusiak, Pawel Adamski, Malgorzata Zwolinska-Wcislo, Tomasz Mach, Piotr B Heczko, Magdalena Strus
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Possible role of Escherichia coli in propagation and perpetuation of chronic inflammation in ulcerative colitis

Magdalena Pilarczyk-Zurek et al. BMC Gastroenterol. .

"VSports最新版本" Abstract

Background: This study investigated a possible role of Escherichia coli in propagation and perpetuation of the chronic inflammation in ulcerative colitis (UC). The lesions of UC are located superficially on the rectal and/or colonic mucosa. It is suggested that the commensal bacteria of the digestive tract may play a role in the pathogenesis of UC. Several studies have demonstrated proliferation of E. coli in the gut of UC patients. An increase in the number of E. coli in the inflamed tissue is most probably related to the abundance of iron ions produced by the bacteria VSports手机版. .

Methods: Colon mucosal biopsies were collected from 30 patients with acute-phase UC, both from tissues with inflammatory changes (n = 30) and unchanged tissue with no inflammatory changes (n = 30) from the same patient. Biopsies were also taken from 16 patients with irritable bowel syndrome diarrhea who comprised the control group. Quantitative and qualitative analysis of the biopsy specimens was performed using culture methods and real-time polymerase chain reaction (PCR). Genotyping of the E. coli isolates was done using pulsed-field gel electrophoresis. Multiplex PCR was used to compare the E V体育安卓版. coli strains for the presence of genes responsible for synthesis of iron acquisition proteins: iroN, iutA, iha, ireA, chuA, and hlyA. .

Results: We demonstrated that there was a significant increase in the number of E. coli at the sites of inflammation in patients with UC compared to the control group (P = 0. 031) V体育ios版. Comparative analysis of the restriction patterns of E. coli isolated from inflammatory and unchanged tissues showed that the local inflammatory changes did not promote specific E. coli strains. There was a significant difference in the frequency of the iroN gene in E. coli isolated from patients with UC as compared to the control group. .

Conclusions: The increase in the numbers of E VSports最新版本. coli in the inflammatory tissues is related to the presence of chuA and iutA genes, which facilitate iron acquisition during chronic intestinal inflammatory processes. .

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Figures

Figure 1
Figure 1
Relative fluorescence is the increase in reporter dye intensity relative to the passive internal reference dye. The amount of E. coli DNA in each sample is shown in the key. The threshold fluorescence, or level at which the threshold cycle was determined, is shown.
Figure 2
Figure 2
Standard curve was generated from the amplification plot in Figure1. y= −1.377 ln(x) + 43.063; correlation coefficient =0.996. Threshold cycle was the cycle number when the threshold fluorescence was reached.
Figure 3
Figure 3
Comparison of restriction patterns of E. coli isolated from patients with active-phase UC. Results based on PFGE. P1/ECA1- P18/ECB1 - strains isolated from UC patients (A-inflammatorily changed place, B – unchanged mucosa, reference strain - Salmonella Braenderup H9812.
Figure 4
Figure 4
Distribution of E. coli with confirmed iroN gene. Specimens isolated from colon mucosa with inflammatory changes and unchanged mucosa from patients with active-phase UC compared with the control group.
Figure 5
Figure 5
α-Hemolysis visible on blood agar.E. coli: P1/ECB2, P8/ECA1, P8/ECB1, P12/ECA2, P12/ECB1, P13/ECB2 – visible type alpha hemolysis, strains: P1/ECA1, P1/ECB1, P6/ECA1, P6/ECB1, P9/ECA1, P9/ECB1 – no alpha hemolysis.
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