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. 2013 Mar-Apr;19(2):69-74.
doi: 10.4103/1319-3767.108474.

Study of the cytoxin-associated gene a (CagA gene) in Helicobacter pylori using gastric biopsies of Iraqi patients

Elham A Kalaf  1 , Zahra M Al-Khafaji, Nahi Y Yassen, Fadel A Al-Abbudi, Saad N Sadwen
Affiliations

Study of the cytoxin-associated gene a (CagA gene) in Helicobacter pylori using gastric biopsies of Iraqi patients

Elham A Kalaf et al. Saudi J Gastroenterol. 2013 Mar-Apr.

"V体育官网" Abstract

Background and aims: The Helicobacter pylori CagA gene is a major virulence factor that plays an important role in gastric pathologies VSports手机版. The size variation of CagA gene, which is dependent on the 3' repeat region, contains one or more Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs and CagA multimerization (CM) motifs. Four segments flanking the EPIYA motifs, EPIYA -A, -B, -C, or -D, were reported to play a crucial role in the pathogenesis of H. pylori infection. The aim was to determine the roles of EPIYA segments and CM motifs in gastroduodenal pathogenesis in an Iraqi population. .

Patients and methods: Gastric biopsies were collected from 210 patients with gastritis, duodenal ulcer (DU), gastric ulcer (GU), and gastric cancer (GC). The EPIYA motif genotyping was determined by polymerase chain reaction and sequencing. The differences in age, gender, and CagA EPIYA motifs of H. pylori between GC, DU, GU and gastritis patients were analyzed using a χ(2) -test. V体育安卓版.

Results: A total of 22 (45. 8%) strains had three copies of EPIYA (ABC type), 2 (4. 16%) had four copies (ABCC type), 6 (12. 7%) had five copies (ABCCC type), 13 (27. 08%) had two copies (AB type), 3 (6. 25%) had the BC, and 2 (4. 17%) had AC motif V体育ios版. The alignment of the deduced protein sequences confirmed that there were no East Asian type EPIYA-D sequences in Iraqi strains. A significant association was found between increase in number of EPIYA-C motifs and GU (P ≤ 0. 01) compared with gastritis. .

Conclusions: The structure of the 3' region of the CagA gene in Iraqi strains was Western type with a variable number of EPIYA-C and CM motifs. A significant association was found between increase in number of EPIYA-C motifs and GU compared with gastritis indicating predictive association with the severity of the disease. The GenBank accession numbers for the partial CagA nucleotide sequences determined in this study are JX164093-JX164112 VSports最新版本. .

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Conflict of interest statement

Conflict of Interest: None declared.

"VSports app下载" Figures

Figure 1
Figure 1
PCR Amplification products of ureC(glmM) gene 296 bp of H. pylori. (M: ladder marker, N: Control negative, Lane 1 to 8 positive results)
Figure 2
Figure 2
PCR Amplification product of CagA gene (5′ conserved region) 349 bp of H. pylori. (Lane M: Ladder marker, Lane 1 to11 biopsy samples; C + positive control, C-, negative control)
Figure 3
Figure 3
PCR products of the CagA 3’ end variable region using primers Cag2-Cag4. Lanes: M, 100-bp DNA marker; 1 to 6 and 8 to12, Single H. pylori strains, 7 mixed isolates
Figure 4
Figure 4
Association between number and type of CagA EPIYA motifs and H. pylori-associated diseases
Figure 5
Figure 5
PCR amplification of CagA 3’ end EPIYA motif from H. pylori strains. Using the forward primer CagA 28F and the reverse primers CagA-P1C (EPIYA A), CagA-P2CG and CagA-P2TA (equimolar mixture; EPIYA- B), CagA-P3E (EPIYA -C) and CagA -PD (EPIYA -D). (1) ABCC motifs, and (2) ABC motifs. M: 100-bp DNA marker
Figure 6
Figure 6
Alignment of partial CagA peptide sequences (showing the EPIYA and CM motifs) from 20 Iraqi H. pylori strains including the H. pylori reference strain 26695 (CM marked with *)
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