VSports在线直播 - Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The VSports app下载. gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2012 Dec 13;4(1):21.
doi: 10.1186/1757-4749-4-21.

"V体育2025版" Mucosa-associated but not luminal Escherichia coli is augmented in Crohn's disease and ulcerative colitis

Helton Luis de Souza  1 , Vanessa R de Carvalho, Fernando Gomes Romeiro, Ligia Yukie Sassaki, Rogeria Keller, Josias Rodrigues
Affiliations

"V体育官网入口" Mucosa-associated but not luminal Escherichia coli is augmented in Crohn's disease and ulcerative colitis

Helton Luis de Souza et al. Gut Pathog. .

Abstract

Background: Escherichia coli is believed to participate in the etiology of Crohn's disease (CD) and possibly of ulcerative colitis (UC), due at least in part to the observed rise in the number of these bacteria in the gut microbiota of CD and UC patients VSports手机版. Nevertheless, it is not fully understood whether this quantitative variation occurs equally throughout the mucosal and luminal spaces of the gut. To assess this question, stools and mucosa biopsies from distinct intestinal sites were cultured aiming at determining their E. coli concentration. The cultures were additionally screened for the presence of some virulence genes of pathogenic E. coli. .

Results: Analyses of clinical materials from 14 controls (38 biopsies and 14 stools samples), 11 CD (25 biopsies and 11 stools samples) and 7 UC patients (18 biopsies and 7 stools samples) indicated no significant variation in the number of E. coli present in stools, but a rise of at least one log10 CFU/mg in biopsies from the ileum of CD patients and the sigmoid and rectum of CD and UC patients. The cultures were screened for the presence of E V体育安卓版. coli attaching and effacing (eae), invasion plasmid antigen H (ipaH), aggregative adherence transcriptional activator (aggR), Shiga cytotoxins (stx), and heat labile enterotoxin (elt) and the following serine proteases autotransporters of Enterobacteriaceae (SPATE) genes: plasmid encoded toxin (pet), secreted autotransporter toxin (sat), Shigella extracellular protein (sepA), protein involved in intestinal colonization (pic) and Shigella IgA-like protease homolog (sigA). Six of the 10 genes screened were detected in the total of samples investigated: aggR, eae, pet, sat, sepA and sigA. No difference in the prevalence of any of these markers was observed in cultures from different clinical materials or groups of patients. .

Methods: Bacterial quantitation was carried out following cultures of diluted samples suspensions in MacConkey agar, Wilkins Chalgren agar for anaerobes, E. coli/coliform chromocult agar, and blood agar. Screening for E. coli virulence genes was performed by multiplex PCR of DNA purified from total MacConkey undiluted broth cultures V体育ios版. .

Conclusion: In CD and UC patients only the mucosa associated population of E. coli is augmented and the proliferation is prominent in the ileum of CD and rectum and sigmoid of both UC and CD patients which are sites where the lesions usually are observed VSports最新版本. The augmented E. coli population in these sites presented a low number of the virulence markers, possibly meaning that they are not relevant for the disease process. .

PubMed Disclaimer

"V体育官网入口" Figures

Figure 1
Figure 1
Average bacterial concentration (CFU/mg) in distinct clinical samples from Controls (white bars), Crohn’s Disease (grey bars) and ulcerative colitis (dark bars) patients.A, Total counting in MacConkey agar cultures; B, Purple colonies counting in brilliance E. coli/coliform agar cultures. IL, RC, LC, S/R, ST, respectively, biopsies from ileum, right colon, left colon, sigmoid and rectum and stools. Asterisks indicate values with significant differences (P<0.05, Paired t-test) in comparisons with controls. See Table 2 for numbers of samples analysed per clinical material.
Figure 2
Figure 2
DNA amplified by multiplex PCR, with primers for Diarrheagenic E.coli typical virulence genes (MDEC), and serine protease autotransporters of Enterobacteriaceae (SPATE) genes (MSPATE). Lanes 1–4, Reference strains; lanes 5–10, Pools of Gram negative bacteria (growth in MacConkey broth) from some clinical samples (see Table 1). Lane 11, 1kb DNA ladder.
See this image and copyright information in PMC

References (V体育官网入口)

    1. Martin HM, Campbell BJ, Hart CA, Mpofu C, Nayar M, Singh R, Englyst H, Williams HF, Rhodes JM. Enhanced Escherichia coli adherence and invasion in Crohn’s disease and colon cancer. Gastroenterology. 2004;127(1):80–93. doi: 10.1053/j.gastro.2004.03.054. - DOI - PubMed
    1. Sokol H, Lepage P, Seksik P, Dore J, Marteau P. Temperature gradient gel electrophoresis of fecal 16S rRNA reveals active Escherichia coli in the microbiota of patients with ulcerative colitis. J Clin Microbiol. 2006;44(9):3172–3177. doi: 10.1128/JCM.02600-05. - DOI - PMC - PubMed
    1. Kotlowski R, Bernstein CN, Sepehri S, Krause DO. High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel disease. Gut. 2007;56(5):669–675. doi: 10.1136/gut.2006.099796. - DOI (V体育官网) - PMC - PubMed
    1. Rhodes JM. The role of Escherichia coli in inflammatory bowel disease. Gut. 2007;56(5):610–612. doi: 10.1136/gut.2006.111872. - DOI - PMC - PubMed
    1. Cartun RW, Van Kruiningen HJ, Pedersen CA, Berman MM. An immunocytochemical search for infectious agents in Crohn’s disease. Mod Pathol. 1993;6(2):212–219. - PubMed (V体育官网入口)

LinkOut - more resources