Background: Angiogenesis is pivotal in tumor development. Vascular endothelial growth factor-A (VEGF-A) is considered one of the most important angiogenic factors, but lately several microRNAs (miRs) have been associated with vascular development. miR-126 has been related to tumor angiogenesis and in the regulation of VEGF-A. The authors aimed to investigate the prognostic impact of miR-126 and its co-expression with VEGF-A in nonsmall cell lung cancer (NSCLC) patients VSports手机版. .

Methods: Tumor tissue samples from 335 resected stage I to IIIA NSCLC patients were obtained and tissue microarrays (TMAs) were constructed with 4 cores from each tumor specimen. VEGF-A expression was evaluated by immunohistochemistry, and in situ hybridization was used to evaluate the expression of miR-126 V体育安卓版. .

Results: In the total material, miR-126 was a significant negative prognostic factor in both univariate (P = . 005) and multivariate analyses (hazard ratio [HR] 1. 8, 95% confidence interval [CI] 1. 2-2. 8, P = . 01). Stratified by histology, miR-126 was only significant in squamous cell carcinomas (univariate: P < . 001; multivariate: HR 3. 1, CI 95% 1 V体育ios版. 7-5. 6, P<. 001). Stratified by lymph node status, miR-126 was significant only in the lymph node-positive subgroup (univariate: P<. 001; multivariate: HR 4. 1, CI 95% 2. 0-8. 4, P < . 001). High miR-126 expression correlated significantly with high VEGF-A expression (P = . 037). The co-expression of miR-126 and VEGF-A had a significant prognostic impact (P = . 002), with 5-year survival rates of 68%, 51%, and 42% for low/low (n = 150), mixed combinations (n = 129), and high/high (n = 35) expression, respectively. .

Conclusions: miR-126 is a strong and independent negative prognostic factor in NSCLC, and its prognostic impact appears related primarily to histology and nodal status. VSports最新版本.