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. 2010 Dec;17(12):1942-3.
doi: 10.1038/cdd.2010.123. Epub 2010 Oct 8.

Cyclophilin A release as a biomarker of necrotic cell death

D E Christofferson, J Yuan

Cyclophilin A release as a biomarker of necrotic cell death

D E Christofferson et al. Cell Death Differ. 2010 Dec.
No abstract available

PubMed Disclaimer

Conflict of interest statement (VSports app下载)

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Cyclophilin A is released from necroptotic cells as a result of cytoplasmic membrane permeabilization. A, L929 cells were treated with 100μM zVAD.fmk for indicated hrs. The supernatants were collected, concentrated, and analyzed by SDS-PAGE followed by western blotting using anti-CypA and anti-HMGB1. B, L929 cells were treated with 100μM zVAD.fmk in the presence or absence of 30μM Nec-1. The cell lysate and supernatant were analyzed by western blotting using anti-CypA. Anti-Tubulin was used as a control. Cell viability was determined by ATP assay and indicated as a bar graph below. C, FADD-deficient Jurkat cells (left panel) or wt Jurkat cells (right panel) were treated with 10ng/ml hTNFα to induce necroptosis, or 10ng/ml hTNFα + 0.5μg/ml CHX to induce apoptosis, respectively. The cell lysate and supernatant were analyzed by western blotting using anti-CypA. The percentages of cell death were determined by ATP assay and indicated below.
See this image and copyright information in PMC

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