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. 2010 Aug 27;33(2):229-40.
doi: 10.1016/j.immuni.2010.08.002.

VSports - Differentiation and persistence of memory CD8(+) T cells depend on T cell factor 1

Xinyuan Zhou  1 , Shuyang Yu, Dong-Mei Zhao, John T Harty, Vladimir P Badovinac, Hai-Hui Xue
Affiliations

Differentiation and persistence of memory CD8(+) T cells depend on T cell factor 1

V体育ios版 - Xinyuan Zhou et al. Immunity. .

"V体育平台登录" Abstract

T cell factor 1 (TCF-1) is a transcription factor known to act downstream of the canonical Wnt pathway and is essential for normal T cell development. However, its physiological roles in mature CD8(+) T cell responses are unknown. Here we showed that TCF-1 deficiency limited proliferation of CD8(+) effector T cells and impaired their differentiation toward a central memory phenotype. Moreover, TCF-1-deficient memory CD8(+) T cells were progressively lost over time, exhibiting reduced expression of the antiapoptotic molecule Bcl-2 and interleukin-2 receptor beta chain and diminished IL-15-driven proliferation. TCF-1 was directly associated with the Eomes allele and the Wnt-TCF-1 pathway was necessary and sufficient for optimal Eomes expression in naive and memory CD8(+) T cells VSports手机版. Importantly, forced expression of Eomes partly protected TCF-1-deficient memory CD8(+) T cells from time-dependent attrition. Our studies thus identify TCF-1 as a critical player in a transcriptional program that regulates memory CD8 differentiation and longevity. .

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Figures

Figure 1
Figure 1. TCF-1 deficiency limits expansion of antigen-specific effector CD8+ T cells
(A) Five hundreds of WT or Tcf7-/- OT-I T cells (CD45.2+) were injected into CD45.1+ B6.SJL recipients, followed by i.v. infection with 5 × 106 CFU actA-LM-Ova. Kinetics of early responses of WT or Tcf7-/- OT-I cells were tracked in the PBLs, and their percentages in CD8+ T cells are shown. Data are representative of 3 independent experiments with similar results (n ≥ 3 for each time point). All p-values, including those in following figures, were determined using Student's t-test. (B) Numbers of effector OT-I T cells in the spleen on day 7 after infection. Data are pooled from 4 independent experiments. (C) Cell surface phenotypes of effector OT-I T cells. Day 7 effector T cells were analyzed for KLRG1, IL-7Rα, CD62L, and CD44 expression. Percentages for KLRG1+, CD44+CD62Llo, and CD44+CD62Lhi populations are shown. Shaded histogram in IL-7Rα denotes its expression in naïve CD8+ T cells for a direct comparison with the effector T cells. (D) Production of effector molecules by effector OT-I T cells. Splenocytes were isolated on day 7 after infection and incubated with Ova peptide for 6 hrs in vitro. The cells were sequentially surface-stained, fixed and permeabilized, and intracellularly stained for IL-2, TNF-α, and granzyme B. Gating of positive populations was based on respective isotype controls. For (C) and (D), data are representative from at least 3 independent experiments with similar results.
Figure 2
Figure 2. Tcf7-/- memory CD8+ T cells are impaired in secondary expansion and in differentiation to a Tcm phenotype
(A) Frequency of early memory OT-I cells in PBLs. B6.SJL recipients of either WT or Tcf7-/- OT-I cells were infected with actA-LM-Ova, and the percentage of CD45.2+ OT-I in CD8+ T cells was determined on days 34-44 after infection. Data are pooled results from 2 independent experiments. (B) Secondary expansion of memory CD8+ T cells in the spleens. Sorted WT or Tcf7-/- memory OT-I T cells (6 × 103) were transferred into naïve B6.SJL hosts, followed by infection with 2 × 105 CFU of virulent LM-Ova. The numbers of CD45.2+ OT-I cells in the spleens were determined 6 days later. Data are means ± s.d. (n = 3). Similar results were obtained when total splenocytes containing equivalent numbers of WT or Tcf7-/- memory OT-I cells were transferred without sorting separation (not shown). (C) In vivo killing capacity of memory CD8+ T cells. CD45.2+ splenocytes were differentially labeled with CFSE. Ova peptide-pulsed CFSElo and non-peptide pulsed CFSEhi cells were injected at 1:1 ratio into naïve or immune chimeras (35 days after infection). The spleens were harvested 4 hrs later, and percentages of CFSElo and CFSEhi cells in CD45.2+ splenocytes were determined. Data are representative of 2 independent experiments with similar results. (D) Surface staining for CD62L, CCR7, IL-7Rβ, and KLRG1 on antigen-specific memory CD8+ T cells. During days 75-85 after infection with actA-LM-Ova, splenocytes from WT or Tcf7-/- OT-I recipients were stained. Percentages of CD62Lhi and CCR7+ subsets were shown in histograms, with shaded histogram denoting isotype control. Data are representative of 2-3 independent experiments (n ≥ 4). (E) Intracellular detection of IFN-γ, IL-2, TNF-α, and granzyme B in memory CD8+ T cells. Splenocytes were stimulated with Ova peptide for 6 hrs, followed by surface and intracellular staining. Gating of positive populations was based on respective isotype controls. All data are representative of 3 independent experiments (n ≥ 5).
Figure 3
Figure 3. Long-term maintenance and IL-15 responsiveness of memory CD8+ T cells depend on TCF-1
(A) Progressive loss of Tcf7-/- memory CD8+ T cells. B6.SJL recipients of WT or Tcf7-/- OT-I cells were sacrificed on indicated days post-infection and memory OT-I T cells in the spleens were enumerated. Data are pooled from at least 3 independent experiments. (B) Tissue distribution of memory CD8+ T cells. During 75-85 days after infection, frequency of memory OT-I in CD8+ T cells was determined in indicated tissues. Data are pooled from 2 independent experiments. (C) Expression of IL-15Rα and IL-2Rβ on memory CD8+ T cells. Memory OT-I cells were surface-stained, and percentages of positive cells were shown in representative histograms based on isotype staining (shaded). Cumulative results from 2-4 experiments were shown on the right panel as ΔMFI, the difference of MFI (mean fluorescent intensity) values of antibody- and isotype-stained entire cell populations without positivity gating. The same approach was used to present data in Figures 3E, 4B, 5E, and 7C. (D) BrdU uptake in memory CD8+ T cells. The B6.SJL recipients were i.p. injected with BrdU on day 70 after infection and fed with BrdU in drinking water for 1 week. The percentage of BrdU+ population was shown in representative contour plots (on the left) or cumulative data from 3 independent experiments (on the right). (E) Expression of Bcl-2 in memory CD8+ T cells. Memory OT-I cells were intracellularly stained for Bcl-2. Data are either representative of or pooled from 3 independent experiments. (F) Proliferation of memory CD8+ T cells in response to IL-15. Memory OT-I cells were labeled with CFSE and cultured in the presence of IL-15 (50 ng/ml). The shaded histogram denotes the CFSE level in cells cultured without IL-15, and the percentage denotes actively dividing cells. Representative data after 4-day culture are shown for 2 independent experiments with similar results (n = 3).
Figure 4
Figure 4. TCF-1 is necessary for optimal Eomes expression
(A) Transcriptomic analysis revealed expression changes in genes that are critical for regulating CD8+ T cell activities. Day 70-80 memory CD8+ T cells were sorted from 3 WT or 3 Tcf7-/- OT-I recipients and subjected to microarray analysis using Mouse GENE 1.0 ST arrays. Heatmaps of select genes and colour-coded scales are shown. (B) Eomes but not T-bet was expressed at lower levels in Tcf7-/- memory CD8+ T cells. Memory CD8+ T cells (>70 days after infection) were intranuclearly stained for Eomes and T-bet. Shown on left are representative histograms with percentages of positive subsets, and on right are cumulative ΔMFI data from 3 independent experiments.
Figure 5
Figure 5. Activation of the canonical Wnt/TCF-1/β-catenin pathway is sufficient to induce Eomes expression
(A) Inhibition of GSK3β induces Eomes expression in memory CD8+ T cells. Day 60 memory OT-I T cells were sorted from LM-Ova-infected B6.SJL recipients and treated with DMSO, BIO-acetoxime (BIO), or N-methylated BIO (MeBIO) for 12 hrs. The expression of select genes was quantitatively determined, and all normalized to the samples treated with DMSO. (B) Induction of Axin2 and Eomes by Wnt3a in naïve CD8+ T cells. Freshly isolated naïve CD8+ T cells from WT or Tcf7-/- mice were exposed to Wnt3a conditioned or control medium for 3 hrs. Gene expression was quantitatively determined and then normalized to the control medium-treated samples. (C) Effect of Wnt and TCR stimulation in naïve CD8+ T cells. WT naïve CD8+ T cells were stimulated with Wnt3a and/or plate-bound anti-CD3 (5 μg/ml) + soluble anti-CD28 (1 μg/ml) for 3 hrs, and gene expression was quantitatively determined and normalized as in (B). (D) Effect of Wnt and TCR stimulation in memory CD8+ T cells. Memory CD8+ T cells were isolated as in (A) and stimulated as in (C), and gene expression was quantitatively determined. For (A)-(D), data are representative of at least 2 independent experiments with similar results (n ≥ 4). (E) Increased Eomes expression in memory CD8+ T cells in the presence of constitutively active Wnt signalling. Double transgenic (dTg) mice with forced expression of p45 TCF-1 and stabilized β-catenin and their WT littermates were infected with actA-LM-Ova. During days 45-55 after infection, memory CD8+ T cells were identified by intracellular detection of IFN-γ after 6-hr Ova peptide stimulation, and Eomes or T-bet expression was determined in IFN-γ+ CD8+ T cells. Data shown are representative histograms (on the left) and cumulative ΔMFI from 2-3 independent experiments (on the right).
Figure 6
Figure 6. TCF-1 is directly associated with regulatory sequences in the Eomes gene
(A) Schematic showing locations of conserved consensus TCF-1 binding motifs in the 5’-regulatory region of the Eomes gene. Multiple species conservation from the UCSC genome browser is shown on the top, and locations of each conserved TCF-1 motif and corresponding PCR amplicon are marked below. The 3 TCF-1 motifs found in -3.5 kb were collectively defined as “cluster a”, and the other 3 relatively scattered motifs were referred to as “element b to d”. See Figure S5 for the sequence alignments among different species. (B) TCF-1 binds to the Eomes regulatory sequences in vivo. Chromatin fragments from WT or Tcf7-/- splenic CD8+ T cells were immunoprecipitated with an anti-TCF-1 antibody. Enrichment of each segment was determined with quantitative PCR and normalized to the Rag2 promoter region. Data are representative of 3 independent ChIP experiments with each sample measured in duplicates or triplicates. (C) TLE-GRG and (D) β-catenin co-occupy Eomes regulatory sequences with TCF-1. Chromatin fragments from splenic CD8+ T cells of WT or β-catenin transgenic mice were immunoprecipitated with TLE-GRG or β-catenin antibody, respectively. Enrichment of each segment was quantitatively measured, and data are representative of 2 independent experiments with similar results.
Figure 7
Figure 7. Forced expression of Eomes protects Tcf7-/- memory CD8+ T cells from attrition
(A) Representative flow profiles of retrovirally transduced effector (day 7) and memory (day 70+) CD8+ T cells. Percentages of GFP+ subsets, infected with either MigR1 control retrovirus or Eomes-expressing retrovirus, in CD8+CD45.2+ cells are shown from 2 independent experiments (n ≥ 4). (B) Fold changes of GFP+ cells during OT-I response. GFP+ percentage in each recipient mouse on day 7 after infection was arbitrarily set to 1, and that in each mouse during the memory phase was normalized to day 7 to calculate the fold changes. Data are means ± s.d. of at least 4 individual recipients for each group (pooled from 2 independent experiments). (C) IL-2Rβ expression in retrovirally infected WT and Tcf7-/- memory CD8+ T cells. Splenocytes were isolated from recipients of retrovirally infected WT or Tcf7-/- OT-I T on day 40 after LM-Ova infection, and IL-2Rβ expression was determined on GFP+CD45.2+CD8+ T cells with representative histograms and cumulative ΔMFIs shown from 2 independent experiments.
See this image and copyright information in PMC

Comment in

  • TCF-1 flips the switch on Eomes.
    Paley MA, Wherry EJ. Paley MA, et al. Immunity. 2010 Aug 27;33(2):145-7. doi: 10.1016/j.immuni.2010.08.008. Immunity. 2010. PMID: 20732636

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